ArticleNature neuroscience2024
Multimodal evaluation of network activity and optogenetic interventions in human hippocampal slices.
Abstract read
In one paragraph
Article in Nature neuroscience, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
0numbers the graph read from it
0cells of the map it votes in
24citing papers in PubMed
–field-weighted citation impact
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
24 citing papers in PubMed.
- Adaptive Network Stabilization Despite Profound Structural Reorganization in Human Organotypic Cultures.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- Loss of schizophrenia risk gene XPO7 disrupts neuronal excitability and network regularity via altered NaMolecular psychiatry · 2026Article
- Article
- Gene therapy for epilepsy: An emerging, promising approach for a serious neurological disorder.Journal of internal medicine · 2026Review
- Article
- Multimodal approach to characterize surgically removed epileptogenic zone from patients with focal drug-resistant epilepsy: From operating room to wet lab.Epilepsia open · 2026Article
- New directions for complex systems in contemporary neuroscience: a morphodynamic and emergent function approach.Frontiers in computational neuroscience · 2026Review
- From sympathetic storm to systemic inflammation: spatiotemporal dynamics of the brain-lung axis in neurogenic pulmonary edema.Frontiers in neuroscience · 2026Review
- CulturedBrain communications · 2026Article
- From mice to clinical relevance: humanizing neuroscience with human-based model systems.Frontiers in cellular neuroscience · 2026Review
- An Active, Multimodal Neural Interface for Real-Time Monitoring of Cortical Electrical, Thermal, and Optical Dynamics.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- Stabilized Ion Selectivity Corrects Activation Drift in Kalium Channelrhodopsins.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- Microscale maps of bursting dynamics across human hippocampal slices from patients with epilepsy.Journal of neurophysiology · 2026Article
- Live-cell physiology in human brain tissue culture-the potential, the challenges, and the lessons learned.Frontiers in cellular neuroscience · 2026Review
- Subarachnoid hemorrhage mediates human neocortical network, membrane potential, and action potential bursting via glutamate receptors.Communications biology · 2025Article
- Article
- Advances in large-scale electrophysiology with high-density microelectrode arrays.Lab on a chip · 2025Review
- Ultrastructural membrane dynamics of mouse and human cortical synapses.bioRxiv : the preprint server for biology · 2025Article
- Incubator-Free Organoid Culture in a Sealed Recirculatory System.bioRxiv : the preprint server for biology · 2025Article
- Pathological microcircuits and epileptiform events in patient hippocampal slices.bioRxiv : the preprint server for biology · 2025Article
Corrections and comments
- Commented on by
Authors and funding
26 authors.
John P Andrews *Department of Neurological Surgery, University of California, San Francisco, San Francisco, CA, USA.ORCID http://orcid.org/0000-0002-8025-1525
Jinghui Geng *Department of Electrical and Computer Engineering, University of California, Santa Cruz, Santa Cruz, CA, USA.
Kateryna Voitiuk *UC Santa Cruz Genomics Institute, University of California, Santa Cruz, Santa Cruz, CA, USA.ORCID http://orcid.org/0000-0002-6392-5188
Matthew A T ElliottUC Santa Cruz Genomics Institute, University of California, Santa Cruz, Santa Cruz, CA, USA.ORCID http://orcid.org/0000-0001-6313-6819
David ShinDepartment of Neurological Surgery, University of California, San Francisco, San Francisco, CA, USA.
Ash RobbinsDepartment of Electrical and Computer Engineering, University of California, Santa Cruz, Santa Cruz, CA, USA.ORCID http://orcid.org/0000-0002-5556-8547
Alex SpaethDepartment of Electrical and Computer Engineering, University of California, Santa Cruz, Santa Cruz, CA, USA.ORCID http://orcid.org/0000-0003-0702-3945
Albert WangDepartment of Neurological Surgery, University of California, San Francisco, San Francisco, CA, USA.ORCID http://orcid.org/0000-0001-5989-4617
Lin LiCalifornia Institute for Quantitative Biosciences, University of California, Berkeley, Berkeley, CA, USA.
Daniel SolisDepartment of Biomolecular Engineering, University of California, Santa Cruz, Santa Cruz, CA, USA.
Matthew G KeefeDepartment of Neurological Surgery, University of California, San Francisco, San Francisco, CA, USA.
Jessica L SevetsonUC Santa Cruz Genomics Institute, University of California, Santa Cruz, Santa Cruz, CA, USA.ORCID http://orcid.org/0000-0001-7434-1447
Julio A Rivera de JesúsDepartment of Bioengineering, University of California, Berkeley, California, CA, USA.
Kevin C DonohueDepartment of Psychiatry and Behavioral Sciences, University of California, San Francisco, San Francisco, CA, USA.ORCID http://orcid.org/0000-0002-4237-5957
H Hanh LarsonDepartment of Pathology, University of California, San Francisco, San Francisco, CA, USA.
Drew EhrlichUC Santa Cruz Genomics Institute, University of California, Santa Cruz, Santa Cruz, CA, USA.
Kurtis I AugusteDepartment of Neurological Surgery, University of California, San Francisco, San Francisco, CA, USA.
Sofie SalamaUC Santa Cruz Genomics Institute, University of California, Santa Cruz, Santa Cruz, CA, USA.ORCID http://orcid.org/0000-0001-6999-7193
Vikaas SohalDepartment of Psychiatry and Behavioral Sciences, University of California, San Francisco, San Francisco, CA, USA.ORCID http://orcid.org/0000-0002-2238-4186
Tal SharfDepartment of Biomolecular Engineering, University of California, Santa Cruz, Santa Cruz, CA, USA.ORCID http://orcid.org/0000-0002-7899-2818
David HausslerDepartment of Electrical and Computer Engineering, University of California, Santa Cruz, Santa Cruz, CA, USA.ORCID http://orcid.org/0000-0003-1533-4575
Cathryn R CadwellDepartment of Neurological Surgery, University of California, San Francisco, San Francisco, CA, USA.ORCID http://orcid.org/0000-0003-1963-8285
David V SchafferCalifornia Institute for Quantitative Biosciences, University of California, Berkeley, Berkeley, CA, USA.ORCID http://orcid.org/0000-0002-9625-0121
Edward F ChangDepartment of Neurological Surgery, University of California, San Francisco, San Francisco, CA, USA. edward.chang@ucsf.edu.ORCID http://orcid.org/0000-0003-2480-4700
Mircea TeodorescuDepartment of Electrical and Computer Engineering, University of California, Santa Cruz, Santa Cruz, CA, USA. mteodore@ucsc.edu.ORCID http://orcid.org/0000-0001-7085-5248
Tomasz Jan NowakowskiDepartment of Neurological Surgery, University of California, San Francisco, San Francisco, CA, USA. tomasz.nowakowski@ucsf.edu.ORCID http://orcid.org/0000-0003-2345-4964
Funding
Nanoparticle Tracking Analyzer (NTA) for the Center for Live Cell GenomicsRM1HG011543 · NHGRI · UNIVERSITY OF CALIFORNIA SANTA CRUZ · PI HAUSSLER, DAVID H, SALAMA, SOFIE REDA · 2021 to 2025
$12.0MPREDOCTORAL TRAINING IN DEVELOPMENTAL BIOLOGYT32HD007470 · NICHD · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI Todd Nystul · 1994 to 2026
$7.7MNINDS Research Education Programs for Residents and Fellows in Neurology, Neurosurgery, Neuropathology, Neuroradiology and Emergency Medicine (R25)R25NS070680 · NINDS · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI JOSEPHSON, SCOTT ANDREW · 2010 to 2023
$6.8MBRAIN CONNECTS: Synaptic Resolution Whole-Brain Circuit Mapping of Molecularly Defined Cell Types Using a Barcoded Rabies VirusU01NS132353 · NINDS · STANFORD UNIVERSITY · PI TOLIAS, ANDREAS, ZADOR, ANTHONY M · 2023 to 2025
$6.1MIRACDA at UCSC and CSUMBK12GM139185 · NIGMS · UNIVERSITY OF CALIFORNIA SANTA CRUZ · PI FORSBERG, CAMILLA, HINCK, LINDSAY E · 2020 to 2024
$4.1MDissecting the role of NOTCH2NL genes in human brain development and neurological disorders associated with chromosome 1q21.1 distal duplications and deletions.R01MH120295 · NIMH · UNIVERSITY OF CALIFORNIA SANTA CRUZ · PI SALAMA, SOFIE REDA · 2020 to 2024
$3.7MMachine Learning Augmented Discovery of AAV Capsids for Cell Type Specific Access into Human Neurons and GliaUF1MH130700 · NIMH · UNIVERSITY OF CALIFORNIA BERKELEY · PI NOWAKOWSKI, TOMASZ, SCHAFFER, DAVID V · 2022 to 2022
$2.9MDevelopmental Timing During Cortical DevelopmentR01NS123263 · NINDS · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI Tomasz Nowakowski · 2022 to 2026
$2.1MUCSC Graduate Program in Genome SciencesT32HG012344 · NHGRI · UNIVERSITY OF CALIFORNIA SANTA CRUZ · PI Angela Norie Brooks, Christopher Vollmers · 2022 to 2026
$1.5MThe role of retinoic acid signaling in patterning the human cerebral cortexK08NS126573 · NINDS · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI Cathryn Rene Cadwell · 2022 to 2026
$1.2MNHGRI NIH HHS RM1 HG011543NHGRI NIH HHS T32 HG012344NICHD NIH HHS T32 HD007470NIGMS NIH HHS K12 GM139185NIMH NIH HHS R01 MH120295NIMH NIH HHS UF1 MH130700NINDS NIH HHS K08 NS126573NINDS NIH HHS R01 NS123263NINDS NIH HHS R25 NS070680NINDS NIH HHS U01 NS132353NSF | BIO | Division of Biological Infrastructure (DBI) NSF 2034037U.S. Department of Health & Human Services | NIH | National Institute of Mental Health (NIMH) R01MH120295U.S. Department of Health & Human Services | NIH | National Institute of Mental Health (NIMH) UF1MH130700U.S. Department of Health & Human Services | NIH | National Institute of Neurological Disorders and Stroke (NINDS) 5R25NS070680-13U.S. Department of Health & Human Services | NIH | National Institute of Neurological Disorders and Stroke (NINDS) K08NS126573U.S. Department of Health & Human Services | NIH | National Institute of Neurological Disorders and Stroke (NINDS) R01NS123263
Abstract
Seizures are made up of the coordinated activity of networks of neurons, suggesting that control of neurons in the pathologic circuits of epilepsy could allow for control of the disease. Optogenetics has been effective at stopping seizure-like activity in non-human disease models by increasing inhibitory tone or decreasing excitation, although this effect has not been shown in human brain tissue. Many of the genetic means for achieving channelrhodopsin expression in non-human models are not possible in humans, and vector-mediated methods are susceptible to species-specific tropism that may affect translational potential. Here we demonstrate adeno-associated virus-mediated, optogenetic reductions in network firing rates of human hippocampal slices recorded on high-density microelectrode arrays under several hyperactivity-provoking conditions. This platform can serve to bridge the gap between human and animal studies by exploring genetic interventions on network activity in human brain tissue.
Indexed as
HippocampusOptogeneticsAction PotentialsAdultDependovirusFemaleGenetic VectorsHumansMaleMiddle AgedNerve NetNeurons
Identifiers
What OpenQuestion holds
Textmetadata
LicenceTDM
Read underepoch 390
Registered trials
None linked
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.