Evidence map›Paper›PMID 39548173›Full record

ArticleScientific reports2024

Interference of small compounds and Mg

Susana Llanos, Bruno Di Geronimo, Ester Casajús, Elena Blanco-Romero, Rafael Fernández-Leiro, Juan Méndez

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Scientific reports, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Susana LlanosDNA Replication Group, Molecular Oncology Programme, Spanish National Cancer Research Center (CNIO), 3 Melchor Fernandez Almagro, 28029, Madrid, Spain. sllanos@cnio.es.
Bruno Di GeronimoSchool of Chemistry and Biochemistry, Georgia Institute of Technology, 901 Atlantic Drive NW, Atlanta, GA, 30332-0400, USA.
Ester CasajúsGenome Integrity and Structural Biology Group, Structural Biology Programme, Spanish National Cancer Research Center (CNIO), 3 Melchor Fernandez Almagro, 28029, Madrid, Spain.
Elena Blanco-RomeroDNA Replication Group, Molecular Oncology Programme, Spanish National Cancer Research Center (CNIO), 3 Melchor Fernandez Almagro, 28029, Madrid, Spain.
Rafael Fernández-LeiroGenome Integrity and Structural Biology Group, Structural Biology Programme, Spanish National Cancer Research Center (CNIO), 3 Melchor Fernandez Almagro, 28029, Madrid, Spain.
Juan MéndezDNA Replication Group, Molecular Oncology Programme, Spanish National Cancer Research Center (CNIO), 3 Melchor Fernandez Almagro, 28029, Madrid, Spain. jmendez@cnio.es.

Funding

Agencia Estatal de Investigación PID2019-106707-RB
6 · The paper itself

Abstract

The COVID-19 pandemic highlighted the need for the rapid development of antiviral therapies. Viral RNA-dependent RNA polymerases (RdRp) are promising targets, and numerous virtual screenings for potential inhibitors were conducted without validation of the identified hits. Here we have tested a set of presumed RdRp inhibitors in biochemical assays based on fluorometric detection of RdRp activity or on the electrophoretic separation or RdRp products. We find that fluorometric detection of RdRp activity is unreliable as a screening method because many small compounds interfere with fluorophore binding to dsRNA, and this effect is enhanced by the Mg

Indexed as

Antiviral AgentsFluorescent DyesMagnesiumRNA-Dependent RNA PolymeraseRNA, Double-StrandedSARS-CoV-2Coronavirus RNA-Dependent RNA PolymeraseCOVID-19COVID-19 Drug TreatmentEnzyme InhibitorsHumansSuraminAntiviral AgentsCoronavirus RNA-Dependent RNA PolymeraseEnzyme InhibitorsFluorescent DyesMagnesiumRNA-Dependent RNA PolymeraseRNA, Double-StrandedSuraminFluorophoreHigh throughput screeningIn vitro RNA polymerization assayRNA-dependent RNA polymeraseSARS-CoV-2Small molecule inhibitors

Identifiers

PMID39548173
PMCPMC11568178

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.