Evidence map›Paper›PMID 39548114›Full record

ArticleScientific reports2024

Enabling biocontained plant virus transmission studies through establishment of an axenic whitefly (Bemisia tabaci) colony on plant tissue culture.

Natalie S Thompson, David Krum, Yun-Ru Chen, Mariela C Torres, Marena A Trauger, Dalton Strike, Zachary Weston, Jane E Polston, Wayne R Curtis

Erratum issuedAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Scientific reports, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Review
  2. Article
4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

9 authors.

Natalie S ThompsonDepartment of Chemical Engineering, The Pennsylvania State University, University Park, PA, 16802, USA.ORCID http://orcid.org/0000-0003-4595-0571
David KrumDepartment of Chemical Engineering, The Pennsylvania State University, University Park, PA, 16802, USA.ORCID http://orcid.org/0000-0003-2059-0653
Yun-Ru ChenDepartment of Chemical Engineering, The Pennsylvania State University, University Park, PA, 16802, USA.ORCID http://orcid.org/0000-0002-5558-7385
Mariela C TorresDepartment of Chemical Engineering, The Pennsylvania State University, University Park, PA, 16802, USA.ORCID http://orcid.org/0000-0002-7868-0742
Marena A TraugerDepartment of Chemical Engineering, The Pennsylvania State University, University Park, PA, 16802, USA.ORCID http://orcid.org/0000-0003-0781-400X
Dalton StrikeDepartment of Chemical Engineering, The Pennsylvania State University, University Park, PA, 16802, USA.ORCID http://orcid.org/0009-0009-1459-4632
Zachary WestonDepartment of Chemical Engineering, The Pennsylvania State University, University Park, PA, 16802, USA.ORCID http://orcid.org/0009-0005-4577-5197
Jane E PolstonDepartment of Plant Pathology, University of Florida, Gainesville, FL, 32611, USA.ORCID http://orcid.org/0000-0002-7858-9375
Wayne R CurtisDepartment of Chemical Engineering, The Pennsylvania State University, University Park, PA, 16802, USA. wrc2@psu.edu.ORCID http://orcid.org/0000-0001-6976-0890

Funding

Bill and Melinda Gates Foundation OPP51589Defense Advanced Research Projects Agency HR0011-17-2-0055National Science Foundation 1543929National Science Foundation 1659497
6 · The paper itself

Abstract

Whiteflies (Bemisia tabaci) and the diseases they transmit are a major detriment to crop yields and a significant contributor to world hunger. The highly evolved interactions of host plant, phloem-feeding insect vector with endosymbionts and persistently transmitted virus represent a tremendous challenge for interdisciplinary study. Presented here is the establishment of a colony of axenic whiteflies on tissue-cultured plants. Efficient colony establishment was achieved by a surface sterilization of eggs laid on axenic phototrophically tissue-cultured plants. The transfer of emerging whiteflies through coupled tissue culture vessels to new axenic plants facilitates robust subculturing and produces hundreds of whitefly adults per month. Whitefly proliferation on more than two dozen plant species is shown as well as in vitro testing of whitefly preference for different plants. This novel multi-organism system provides the high-level of biocontainment required by Federal permitting to conduct virus transmission experiments. Axenic whitefly adults were able to acquire and transmit a begomovirus into tissue-cultured plants, indicating that culturable gut microorganisms are not required for virus transmission. The approach described enables a wide range of hypotheses regarding whitefly phytopathology without the expense, facilities, and contamination ambiguity associated with current approaches.

Indexed as

HemipteraPlant DiseasesAnimalsAxenic CultureBegomovirusInsect VectorsPlant VirusesTissue Culture TechniquesBegomovirusBiocontainmentGeminivirusin vitroPlant-insect-virus interactionToMoVWhitefly hostWhitefly transmission

Identifiers

PMID39548114
PMCPMC11568280

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.