Evidence map›Paper›PMID 39546197›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2025

Collection and Processing of Samples for Next-Generation Sequencing to Study the Gut Microbiome.

Maxwell Bokor, Emily Chiacchiaro, Sangita Phadtare

Abstract read
PubMed Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

Maxwell BokorDepartment of Biomedical Sciences, Cooper Medical School of Rowan University, Camden, NJ, USA.
Emily ChiacchiaroDepartment of Biomedical Sciences, Cooper Medical School of Rowan University, Camden, NJ, USA.
Sangita PhadtareDepartment of Biomedical Sciences, Cooper Medical School of Rowan University, Camden, NJ, USA. phadtare@rowan.edu.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

16S rRNA gene sequencing is commonly used for identification and quantitation of microorganisms in complex biological mixtures, such as the human gut microbiome. The 16S rRNA gene is an excellent target gene for sequencing DNA in a heterogenous sample, as it is a highly conserved part of the transcriptional machinery. Universal PCR primers are used to amplify the conserved regions of 16S. Thus, it is possible to amplify the gene in a wide range of different microorganisms from a single sample. As the 16S rRNA gene consists of both conserved and variable regions, sequencing of the variable regions can be used to differentiate between different bacterial species.

Indexed as

DNA, BacterialGastrointestinal MicrobiomeHigh-Throughput Nucleotide SequencingRNA, Ribosomal, 16SBacteriaHumansPolymerase Chain ReactionSequence Analysis, DNASpecimen HandlingDNA, BacterialRNA, Ribosomal, 16S16S rRNA sequencingBar coded primersGut microbiomeNucleic acid stabilizer

Identifiers

PMID39546197

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.