Evidence map›Paper›PMID 39545962›Full record

ArticleBioprocess and biosystems engineering2025

Deep eutectic solvents assisted laccase pretreatment for improving enzymatic hydrolysis of corn stover.

Kexin Lin, Weiting Zhang, Xinyang Fan, Xiaoyan Li, Nuomeng Wang, Shuyu Yu, Lei Lu

Abstract read
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In one paragraph

Article in Bioprocess and biosystems engineering, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Kexin LinCollege of Life Sciences, Northeast Forestry University, No. 26, Hexing Road, Harbin, 150040, China.
Weiting ZhangCollege of Life Sciences, Northeast Forestry University, No. 26, Hexing Road, Harbin, 150040, China.
Xinyang FanCollege of Life Sciences, Northeast Forestry University, No. 26, Hexing Road, Harbin, 150040, China.
Xiaoyan LiCollege of Life Sciences, Northeast Forestry University, No. 26, Hexing Road, Harbin, 150040, China.
Nuomeng WangCollege of Life Sciences, Northeast Forestry University, No. 26, Hexing Road, Harbin, 150040, China.
Shuyu YuCollege of Water Conservancy and Civil Engineering, Inner Mongolia Agricultural University, Hohhot, 010018, China. shuyuyu@126.com.
Lei LuCollege of Life Sciences, Northeast Forestry University, No. 26, Hexing Road, Harbin, 150040, China. llu@nefu.edu.cn.

Funding

the Fundamental Research Funds for the Central Universities 2572021BD05the Major Science and Technology Project of Ordos City 2022EEDSKJZDZX014the Planned Science-Technology Project of Inner Mongolia Autonomous Region 2022YFXZ0037
6 · The paper itself

Abstract

The efficient and eco-friendly removal of lignin is a critical challenge for bioethanol production from lignocellulosic biomass. Herein, we report the integration of laccase with deep eutectic solvents (DESs) for the pretreatment of corn stover to enhance the production of reducing sugars. Three betaine-based DESs were prepared and tested for their effects on the activity and stability of a bacterial laccase from Bacillus amyloliquefaciens LC02. The aqueous solution of DESs showed no adverse influence on laccase activity, and the laccase thermostability was improved in the presence of DESs. More than 95% of the laccase activity was retained in the DESs solution during the first hour of incubation at 70 °C. A red shift in the fluorescence spectra was observed for the laccase in the presence of DESs, indicating conformational changes. The laccase was able to degrade a dimeric lignin model compound by cleaving its β-O-4 bond. The transformation products were identified using LC-MS. The maximal lignin removal from corn stover was achieved by pretreatment using laccase in combination with the betaine-glycerol DES, which also resulted in a yield of fermentable sugar that was 130% higher than the control. This combination strategy provides guidance on the application of laccase and DESs in the pretreatment of lignocellulosic biomass.

Indexed as

Bacillus amyloliquefaciensBacterial ProteinsDeep Eutectic SolventsLaccaseLigninZea maysBiomassHydrolysisBacterial ProteinsDeep Eutectic SolventsLaccaseLigninlignocelluloseCorn stoverDeep eutectic solventsDelignificationLaccasePretreatment

Identifiers

PMID39545962

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.