ArticleOpen veterinary journal2024
Zinc-based fixative as a novel approach for histological preservation: A comparative study with formalin-based fixatives.
Article in Open veterinary journal, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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3 authors.
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Abstract
Background: Formalin is a widely used histological fixative despite its carcinogenic properties, inadequate nucleic acid preservation, and prolonged fixation time. Aim: The study intended to prepare a novel zinc-based fixative that is, formalin-free, and cost-effective, provides optimal preservation, with rapid penetration rates, and enhanced Hematoxylin/Eosin-stained sections. Methods: Liver, kidney, brain, and small intestine specimens were collected from 10 adult laboratory rats ( Results: A higher penetration rate was observed with the EZAT solution at 6 and 12 hours' time and the samples reached optimal fixation after 24 hours; with an accelerated diffusion coefficient, and a minimal shrinking effect compared to formalin 10% and NBF. The microscopic evaluation of hematoxylin/eosin-stained sections revealed better staining affinity, refined histological details, and enhanced cytoplasmic and nuclear properties. The overall structural evaluation revealed an excellent microscopic appearance with the EZAT solution compared to formalin-based fixatives. Conclusion: The EZAT fixative should be considered as an everyday preservative in histology and histopathology laboratories. Future studies should be focused on the potential of EZAT in cellular histochemistry and immunohistochemistry practices.
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