ArticlePest management science2025
Cry1 resistance in a CRISPR/Cas9-mediated HaCad1 gene knockout strain of the Australian cotton bollworm Helicoverpa armigera conferta (Lepidoptera: Noctuidae).
Article in Pest management science, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.
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Who cites it
5 citing papers in PubMed.
- Harnessing next-generation molecular technologies for precision control of Tuta absoluta and Helicoverpa armigera in tomato.Functional & integrative genomics · 2026Review
- Harnessing CRISPR-Cas technology for insect pest control: current advances and future perspectives.Molecular biology reports · 2026Review
- Isoform-resolved transcriptomics reveals stage-specific isoform switching and protein domain remodeling inTranscription · 2026Article
- Review
- Functional redundancy in the toxic pathway of Bt protein Cry1Ab, but not Cry1Fa, against the Asian corn borer.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
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7 authors.
Funding
Abstract
backgroundHelicoverpa armigera is a highly polyphagous species that causes huge losses to agricultural and horticultural crops worldwide. In the cotton industry, H. armigera, including the Australian subspecies Helicoverpa armigera conferta, is largely managed using genetically modified crops that express insecticidal toxins, such as Cry1Ac. Resistance to Cry1 proteins occurs and, in some cases, is mediated by changes to HaCad1, a gene that encodes the midgut protein cadherin. Around the world, numerous resistance-associated polymorphisms have been identified in the HaCad1 gene of H. armigera, but Cry1Ac resistance is rare in the Australian subspecies. We used CRISPR/Cas9 to disrupt the cadherin gene in H. armigera conferta and characterised the resulting phenotype with bioassays and transcriptomics.
resultsCompared to the parental strain, the newly generated HaCad1 knockout strain is 44-fold and 16-fold more resistant to Cry1Ac and Cry1A.105, respectively, while wild-type and knockout insects were equally insensitive to Cry1F.
conclusionThe disruption of the HaCad1 gene causes Cry1Ac resistance in Australian H. armigera conferta. However, Cry1Ac resistance remains rare in Australian field populations suggesting that Australia's approach to pest management in cotton has prevented widespread Cry1Ac resistance. © 2024 CSIRO. Pest Management Science published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry.
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