Evidence map›Paper›PMID 39542957›Full record

ArticleRice (New York, N.Y.)2024

OsIAA23 Promotes Heading by Directly Downregulating Ghd7 in rice.

Jia Zhang, Wei Hu, Qingli Wen, Xiaowei Fan, Yong Hu, Qin He, Li Lu, Jinfeng Li, Yongzhong Xing

Abstract read
In one paragraph

Article in Rice (New York, N.Y.), 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Jia Zhang *National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Hongshan Laboratory, Wuhan, 430070, China.
Wei Hu *National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Hongshan Laboratory, Wuhan, 430070, China.
Qingli WenNational Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Hongshan Laboratory, Wuhan, 430070, China.
Xiaowei FanNational Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Hongshan Laboratory, Wuhan, 430070, China.
Yong HuNational Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Hongshan Laboratory, Wuhan, 430070, China.
Qin HeNational Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Hongshan Laboratory, Wuhan, 430070, China.
Li LuNational Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Hongshan Laboratory, Wuhan, 430070, China.
Jinfeng LiCollege of Life Science, Jiangxi Normal University, Nanchang, 330022, China.
Yongzhong XingNational Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Hongshan Laboratory, Wuhan, 430070, China. yzxing@mail.hzau.edu.cn.

Funding

Earmarked Fund for China Agriculture Research System CARS-01High Quality Development Projects of Hubei Province for Seed Industry HBZY2023B001-03Natural Science Foundation of China 32101750Natural Science Foundation of China U20A2031
6 · The paper itself

Abstract

Ghd7 is a central regulator to multiple growth and development processes in rice. While it is not clear how Ghd7 is regulated by upstream factors. To identify its upstream regulator, the truncated Ghd7 promoter fragments were used to screen cis elements binding to rice total nuclear proteins. Electrophoretic mobility shift assays screened one truncated fragment f3 binding to the proteins. Subsequently, the fragment f3 was employed to screen a yeast one-hybrid library, and a transcription factor OsIAA23 was screened as a direct upstream regulator of Ghd7. Dual-luciferase transient assay demonstrated the transcriptional repression effect of OsIAA23 on the activity of Ghd7, and the location of the cis elements binding to OsIAA23 in the region 1264 to 1255 bp upstream of ATG. Genetic analysis between the wild type Ghd7-OsIAA23 and single/double mutants further verified that OsIAA23 downregulated Ghd7 expression and led to a delayed heading under long day conditions. Moreover, natural variations in fragment f3 were associated with heading and geographic distribution in rice. This study sheds light on the direct regulatory mechanism of OsIAA23 on Ghd7, which enriches the understanding of the Ghd7 involved flowering regulatory network in rice.

Indexed as

Cis elementOsIAA23Transcriptional suppressionTruncated promoter fragmentsUpstream regulator

Identifiers

PMID39542957
PMCPMC11564490

What OpenQuestion holds

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LicenceCC BY-NC-ND
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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.