ArticleProceedings of the National Academy of Sciences of the United States of America2024
Cotranslational molecular condensation of cochaperones and assembly factors facilitates axonemal dynein biogenesis.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.
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Who cites it
2 citing papers in PubMed.
- A High-Resolution Subcellular Map of Proteins in Cells with Motile Cilia.Journal of proteome research · 2026Article
- DynAPs and cytoplasmic assembly of axonemal dyneins.Journal of cell science · 2025Article
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Authors and funding
10 authors.
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Abstract
Axonemal dynein, the macromolecular machine that powers ciliary motility, assembles in the cytosol with the help of dynein axonemal assembly factors (DNAAFs). These DNAAFs localize in cytosolic foci thought to form via liquid-liquid phase separation. However, the functional significance of DNAAF foci formation and how the production and assembly of multiple components are so efficiently coordinated, at such enormous scale, remain unclear. Here, we unveil an axonemal dynein production and assembly hub enriched with translating heavy chains (HCs) and DNAAFs. We show that mRNAs encoding interacting HCs of outer dynein arms colocalize in cytosolic foci, along with nascent HCs. The formation of these mRNA foci and their colocalization relies on HC translation. We observe that a previously identified DNAAF assembly, containing the DNAAF Lrrc6 and cochaperones Ruvbl1 and Ruvbl2, colocalizes with these HC foci, and is also dependent on HC translation. We additionally show that Ruvbl1 is required for the recruitment of Lrrc6 into the HC foci and that both proteins function cotranslationally. We propose that these DNAAF foci are anchored by stable interactions between translating HCs, ribosomes, and encoding mRNAs, followed by cotranslational molecular condensation of cochaperones and assembly factors, providing a potential mechanism that coordinates HC translation, folding, and assembly at scale.
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