Evidence map›Paper›PMID 39541307›Full record

ArticlePloS one2024

Effect of laser photobiomodulation combined with hydroxyapatite nanoparticles on the osteogenic differentiation of mesenchymal stem cells using artificial intelligence: An in vitro study.

Eloiza Leonardo de Melo, Jéssica Meirinhos Miranda, Vanessa Bastos de Souza Rolim Lima, Wyndly Daniel Cardoso Gaião, Braulio de Vilhena Amorim Tostes, Claudio Gabriel Rodrigues, Márcia Bezerra da Silva, Severino Alves Júnior, Edson Luiz Pontes Perger, Mávio Eduardo Azevedo Bispo and 1 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in PloS one, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

Eloiza Leonardo de MeloDepartment of Biophotonics in Health Sciences, University of Pernambuco, Recife, Pernambuco, Brazil.
Jéssica Meirinhos MirandaDepartment of Biophotonics in Health Sciences, University of Pernambuco, Recife, Pernambuco, Brazil.ORCID 0000-0003-2882-5103
Vanessa Bastos de Souza Rolim LimaDepartment of Biophotonics in Health Sciences, University of Pernambuco, Recife, Pernambuco, Brazil.
Wyndly Daniel Cardoso GaiãoDepartment of Biophysics and Radiobiology, Federal University of Pernambuco, Recife, Pernambuco, Brazil.ORCID 0000-0001-9219-2414
Braulio de Vilhena Amorim TostesDepartment of Basic Chemistry, Federal University of Pernambuco, Recife, Pernambuco, Brazil.
Claudio Gabriel RodriguesDepartment of Biophysics and Radiobiology, Federal University of Pernambuco, Recife, Pernambuco, Brazil.
Márcia Bezerra da SilvaDepartment of Biophysics and Radiobiology, Federal University of Pernambuco, Recife, Pernambuco, Brazil.
Severino Alves JúniorDepartment of Basic Chemistry, Federal University of Pernambuco, Recife, Pernambuco, Brazil.
Edson Luiz Pontes PergerDepartment of Bioprocess and Biotechnology, Paulista State University, Botucatu, São Paulo, Brazil.
Mávio Eduardo Azevedo BispoDepartment of Computer Science, University of Pernambuco, Recife, Pernambuco, Brazil.
Marleny Elizabeth Márquez de Martínez GerbiDepartment of Biophotonics in Health Sciences, University of Pernambuco, Recife, Pernambuco, Brazil.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

aimTo evaluate in vitro the effect of laser photobiomodulation (PBM) combined or not with 30-nm hydroxyapatite nanoparticles (HANp), on the osteogenic differentiation of human umbilical cord mesenchymal stem cells (hUC-MSCs) by morphometric analysis using artificial intelligence programs (TensorFlow and ArcGIS).

methodsUC-MSCs were isolated and cultured until 80% confluence was reached. The cells were then plated according to the following experimental groups: G1 -control (DMEM), G2 -BMP-2, G3 -BMP-7, G4 -PBM (660 nm, 10 mW, 2.5 J/cm2, spot size of 0.08 cm2), G5 -HANp, G6 -HANp + PBM, G7 -BMP-2 + PBM, and G8 -BMP-7 + PBM. The MTT assay was used to analyze cell viability at 24, 48 and 72 h. Osteogenic differentiation was assessed by Alizarin Red staining after 7, 14 and 21 days. For morphometric analysis, areas of osteogenic differentiation (pixel2) were delimited by machine learning using the TensorFlow and ArcGIS 10.8 programs.

resultsThe results of the MTT assay showed high rates of cell viability and proliferation in all groups when compared to control. Morphometric analysis revealed a greater area of osteogenic differentiation in G5 (HANp = 142709,33±36573,39) and G6 (HANp + PBM = 125452,00±24226,95) at all time points evaluated.

conclusionIt is suggested that HANp, whether combined with PBM or not, may be a promising alternative to enhance the cellular viability and osteogenic differentiation of hUC-MSCs.

Indexed as

Artificial IntelligenceCell DifferentiationDurapatiteLow-Level Light TherapyMesenchymal Stem CellsNanoparticlesOsteogenesisCells, CulturedCell SurvivalHumansDurapatite

Identifiers

PMID39541307
PMCPMC11563392

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.