Evidence map›Paper›PMID 39541046›Full record

ArticleMolecular biology reports2024

LINC00470 promotes malignant progression of testicular germ cell tumors.

Zhizhong Liu, Shanshan Lv, Zailong Qin, Jinhui Shu, Fang Zhu, Yanwei Luo, Liqing Fan, Mengqian Chen, Hao Bo, Lvjun Liu

Abstract read
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In one paragraph

Article in Molecular biology reports, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Review
  2. Review
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Zhizhong Liu *Department of Urology, Hunan Cancer Hospital, The Affiliated Cancer Hospital of Xiangya School of Medicine, Central South University, Changsha, Hunan, China.
Shanshan Lv *Department of Blood Transfusion, The Third Xiangya Hospital of Central South University, Changsha, Hunan, China.
Zailong Qin *Laboratory of Genetics and Metabolism, Guangxi Birth Defects Research and Prevention Institute, Maternal and Child Health Hospital of Guangxi Zhuang Autonomous Region, Nanning, Guangxi, China.
Jinhui ShuReproductive Medicine Center, Maternal and Child Health Hospital of Guangxi Zhuang Autonomous Region, Nanning, Guangxi, China.
Fang ZhuNHC Key Laboratory of Human Stem Cell and Reproductive Engineering, Institute of Reproductive and Stem Cell Engineering, School of Basic Medical Science, Central South University, Changsha, Hunan, China.
Yanwei LuoDepartment of Blood Transfusion, The Third Xiangya Hospital of Central South University, Changsha, Hunan, China.
Liqing FanNHC Key Laboratory of Human Stem Cell and Reproductive Engineering, Institute of Reproductive and Stem Cell Engineering, School of Basic Medical Science, Central South University, Changsha, Hunan, China.
Mengqian ChenDepartment of Drug Discovery and Biomedical Sciences, University of South Carolina, Columbia, SC, 29208, USA.
Hao BoNHC Key Laboratory of Human Stem Cell and Reproductive Engineering, Institute of Reproductive and Stem Cell Engineering, School of Basic Medical Science, Central South University, Changsha, Hunan, China. bohao1990@163.com.
Lvjun LiuHunan Provincial Key Laboratory of Regional Hereditary Birth Defects Prevention and Control, Center of Reproductive Medicine, Changsha Hospital for Maternal & Child Health Care Affiliated to Hunan Normal University, Changsha, Hunan, China. 303374548@qq.com.

Funding

Guangxi Key Laboratory of Reproductive Health and Birth Defects Prevention and Control GXWCH-ZDKF-2022-03Hunan Provincial Key Laboratory of Regional Hereditary Birth Defects Prevention and Control Grant No. HPKL2023011the Natural Science Foundation of Hunan Province 2023JJ60329the Research Program of Hunan Provincial Health Commission 202204055545
6 · The paper itself

Abstract

backgroundTesticular germ cell tumor (TGCT) is a common malignant tumor in adolescents. Now, many long non-coding RNAs (LncRNAs) have been found to have an important function in TGCT. LINC00470 is specifically and highly expressed in TGCT, however, there is still no definite information concerning its role and underlying mechanism in TGCT. The purpose of this research was to look into the involvement of LINC00470 in TGCT and its intrinsic mechanism. METHODS AND

resultsUCSC and GEPIA2 databases were used to analyze the expression of LINC00470, and the BEST website was used to perform GSEA enrichment analysis, immune infiltration analysis, and drug susceptibility analysis. SiRNA transfection was used to silence LINC00470 in TCAM-2 and NCCIT cells. Clone formation and Transwell assays were performed in TGCT cells to confirm the effects of LINC00470 on clone formation, migration, and invasion. Western Blot was performed to determine the expression of proteins related to the EMT and AKT signaling pathways. LINC00470 was specifically highly expressed in TGCT, and played a role in promoting tumor cell clone formation and cell metastasis by affecting the TGF-β and PI3K-AKT-mTOR signaling pathways to regulate the epithelial-mesenchymal transition (EMT) process; LINC00470 may also play a pro-tumor role by negatively regulating immune infiltration; in addition, the expression of LINC00470 was negatively correlated with the chemosensitivity of cisplatin in TGCT patients.

conclusionsLINC00470 may play a significant role in the etiology and metastasis of TGCT through EMT and AKT-mediated signaling pathways.

Indexed as

Cell MovementEpithelial-Mesenchymal TransitionGene Expression Regulation, NeoplasticNeoplasms, Germ Cell and EmbryonalRNA, Long NoncodingSignal TransductionTesticular NeoplasmsCell Line, TumorCell ProliferationDisease ProgressionHumansMalePhosphatidylinositol 3-KinasesProto-Oncogene Proteins c-aktTOR Serine-Threonine KinasesTransforming Growth Factor betaPhosphatidylinositol 3-KinasesProto-Oncogene Proteins c-aktRNA, Long NoncodingTOR Serine-Threonine KinasesTransforming Growth Factor betaAKTCell metastasisEpithelial-mesenchymal transitionImmune responseLINC00470Testicular germ cell tumor

Identifiers

PMID39541046

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.