Evidence map›Paper›PMID 39540743›Full record

ArticleApplied and environmental microbiology2024

Inside the Atacama Desert: uncovering the living microbiome of an extreme environment.

Alexander Bartholomäus, Steffi Genderjahn, Kai Mangelsdorf, Beate Schneider, Pedro Zamorano, Samuel P Kounaves, Dirk Schulze-Makuch, Dirk Wagner

Abstract read
In one paragraph

Article in Applied and environmental microbiology, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.

0numbers the graph read from it
0cells of the map it votes in
9citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

9 citing papers in PubMed.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Alexander Bartholomäus *GFZ German Research Centre for Geosciences, Section Geomicrobiology, Potsdam, Germany.ORCID 0000-0003-0970-7304
Steffi Genderjahn *GFZ German Research Centre for Geosciences, Section Geomicrobiology, Potsdam, Germany.
Kai MangelsdorfGFZ German Research Centre for Geosciences, Section Organic Geochemistry, Potsdam, Germany.
Beate SchneiderGFZ German Research Centre for Geosciences, Section Geomicrobiology, Potsdam, Germany.
Pedro ZamoranoLaboratorio de Microorganismos Extremófilos, University of Antofagasta, Antofagasta, Chile.
Samuel P KounavesDepartment of Chemistry, Tufts University, Medford, Massachusetts, USA.
Dirk Schulze-MakuchGFZ German Research Centre for Geosciences, Section Geomicrobiology, Potsdam, Germany.
Dirk WagnerGFZ German Research Centre for Geosciences, Section Geomicrobiology, Potsdam, Germany.ORCID 0000-0001-5064-497X

Funding

EC | European Research Council (ERC) 339231
6 · The paper itself

Abstract

The Atacama Desert in Chile is one of the driest and most inhospitable places on Earth. To analyze the diversity and distribution of microbial communities in such an environment, one of the most important and challenging steps is DNA extraction. Using commercial environmental DNA extraction protocols, a mixture of living, dormant, and dead cells of microorganisms is extracted, but separation of the different DNA pools is almost impossible. To overcome this problem, we applied a novel method on soils across a west-east moisture transect in the Atacama Desert to distinguish between extracellular DNA (eDNA) and intracellular DNA (iDNA) at the cell extraction level. Here, we show that a large number of living and potentially active microorganisms, such as

Indexed as

BacteriaDesert ClimateExtreme EnvironmentsMicrobiotaSoil MicrobiologyChileDNA, BacterialSoilDNA, BacterialSoilAtacama DesertDNA extractionextracellular DNAintracellular DNAmicrobial diversitymicrobiomespecialist

Identifiers

PMID39540743
PMCPMC11653780

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.