Evidence map›Paper›PMID 39540352›Full record

ArticleMolecular medicine reports2025

Semiquantitative analysis of protein expression in heated rat lens using shotgun proteomics.

Hiroko Otake, Shuya Masuda, Tetsushi Yamamoto, Yoshiki Miyata, Yosuke Nakazawa, Naoki Yamamoto, Atsushi Taga, Hiroshi Sasaki, Noriaki Nagai

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In one paragraph

Article in Molecular medicine reports, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Hiroko Otake *Faculty of Pharmacy, Kindai University, Higashiosaka, Osaka 577‑8502, Japan.
Shuya Masuda *Faculty of Pharmacy, Kindai University, Higashiosaka, Osaka 577‑8502, Japan.
Tetsushi YamamotoFaculty of Pharmacy, Kindai University, Higashiosaka, Osaka 577‑8502, Japan.
Yoshiki MiyataFaculty of Pharmaceutical Sciences, Teikyo University, Tokyo 173‑8606, Japan.
Yosuke NakazawaFaculty of Pharmacy, Keio University, Tokyo 105‑8512, Japan.
Naoki YamamotoResearch Promotion Headquarters, Fujita Health University, Toyoake, Aichi 470‑1192, Japan.
Atsushi TagaFaculty of Pharmacy, Kindai University, Higashiosaka, Osaka 577‑8502, Japan.
Hiroshi SasakiDepartment of Ophthalmology, Kanazawa Medical University, Uchinada, Ishikawa 920‑0293, Japan.
Noriaki NagaiFaculty of Pharmacy, Kindai University, Higashiosaka, Osaka 577‑8502, Japan.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Previous studies have reported that a strong correlation between the estimated cumulative thermal exposure in the crystalline lens and the incidence of nuclear cataracts; however, the precise relationship between temperature and cataracts remains to be fully elucidated. In the present study, the shotgun liquid chromatography/mass spectroscopy‑based global proteomic approach was applied to investigate cataract‑inducing factors in lens cultured at normal (35.0˚C) and slightly warmer (37.5˚C) conditions. In the rat lens, 190 proteins (total) were identified. Of these, 48 proteins (25.3%) were found in lenses cultured at both 35.0˚C and 37.5˚C. Moreover, 85 proteins (44.7%) were unique to lenses cultured at 35.0˚C, while 57 proteins (30.0%) were unique to lenses cultured at 37.5˚C. Protein expression changes in rat lenses cultured at 37.5˚C were examined using a label‑free semiquantitative approach that uses spectral counting and Gene Ontology analysis. Filensin and vimentin protein expression, key factors in maintaining lens structure, were decreased. These findings may serve as a valuable indicator for elucidating the relationship between temperature and the onset of nuclear cataracts.

Indexed as

Eye ProteinsLens, CrystallineProteomicsAnimalsCataractChromatography, LiquidGene OntologyHot TemperatureIntermediate Filament ProteinsMaleProteomeRatsVimentinEye ProteinsfilensinIntermediate Filament ProteinsProteomeVimentincataractlensproteinshotgun proteomicstemperature

Identifiers

PMID39540352
PMCPMC11576961

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.