Evidence map›Paper›PMID 39538093›Full record

ArticleMetabolomics : Official journal of the Metabolomic Society2024

Fast DPPH antioxidant activity analysis by UHPLC-HRMS combined with chemometrics of tempeh during food processing.

Ayu Septi Anggraeni, Anjar Windarsih, Navista Sri Octa Ujiantari, Indrawati Dian Utami, Lucky Prabowo Miftachul Alam, Yuniar Khasanah, Anastasia Wheni Indrianingsih, Suratno

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Article in Metabolomics : Official journal of the Metabolomic Society, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Ayu Septi AnggraeniResearch Center for Food Technology and Processing (PRTPP), National Research and Innovation Agency (BRIN), Yogyakarta, 55861, Indonesia.
Anjar WindarsihResearch Center for Food Technology and Processing (PRTPP), National Research and Innovation Agency (BRIN), Yogyakarta, 55861, Indonesia.
Navista Sri Octa UjiantariDepartment of Pharmaceutical Chemistry, Faculty of Pharmacy, Universitas Gadjah Mada, Yogyakarta, 55281, Indonesia.
Indrawati Dian UtamiDirectorate of Laboratory Management, Research Facilities, and Science and Technology Park, National Research and Innovation Agency (BRIN), Yogyakarta, 55861, Indonesia.
Lucky Prabowo Miftachul AlamDirectorate of Laboratory Management, Research Facilities, and Science and Technology Park, National Research and Innovation Agency (BRIN), Yogyakarta, 55861, Indonesia.
Yuniar KhasanahResearch Center for Food Technology and Processing (PRTPP), National Research and Innovation Agency (BRIN), Yogyakarta, 55861, Indonesia.
Anastasia Wheni IndrianingsihResearch Center for Food Technology and Processing (PRTPP), National Research and Innovation Agency (BRIN), Yogyakarta, 55861, Indonesia.
SuratnoResearch Center for Food Technology and Processing (PRTPP), National Research and Innovation Agency (BRIN), Yogyakarta, 55861, Indonesia. nanosan80@gmail.com.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

introductionTempeh is an antioxidant-rich soybean fermentation product from Java, Indonesia. Cooking methods have an impact on the nutritional value and bioactivity of food.

objectiveThis study aims to investigate how the cooking process affects the metabolites and antioxidant activity in tempeh using ultra-high-performance liquid chromatography-high resolution mass spectrometry (UHPLC-HRMS).

methodsA nontargeted UHPLC-HRMS metabolomics and chemometric analysis were used to evaluate metabolite profiles and antioxidant activity changes because of food processing in tempeh.

resultsThe score plots of tempeh produced by boiling and frying methods displayed a distinct separation from raw tempeh, revealing that the cooking process altered the metabolite composition of tempeh. Due to processing, L-glutamic acid, L-pyroglutamic acid, DL-glutamine, and D-( +)-proline became the most affected metabolites on tempeh. There were 70 metabolites that showed antioxidant activity using the DPPH assay; 23 metabolites significantly differ from DPPH and control for antioxidant activity for all processing tempeh. Metabolites with significantly different antioxidant activity in raw and processed tempeh were dominated by flavonoids, vitamin E, and bioactive lipids.

conclusionThe DPPH antioxidant assay using UHPLC-HRMS is promising as a fast antioxidant assay by simplifying the conventional DPPH antioxidant assay. Further, it can be used to identify the name of metabolites responsible for its antioxidant activity.

Indexed as

AntioxidantsBiphenyl CompoundsFood HandlingMass SpectrometryMetabolomicsChemometricsChromatography, High Pressure LiquidFermentationGlycine maxPicratesSoy Foods1,1-diphenyl-2-picrylhydrazylAntioxidantsBiphenyl CompoundsPicratesAntioxidant activitiesDPPHFood processingMetaboliteTempeh

Identifiers

PMID39538093

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.