Evidence map›Paper›PMID 39537718›Full record

ArticleScientific reports2024

Elimination of olfactory sensory neurons by zinc sulfate inoculation prevents SARS-CoV-2 infection of the brain in K18-hACE2 transgenic mice.

Ji-Hun Lee, Eun-Seon Yoo, Na-Won Kim, Won-Yong Shim, Han-Bi Jeong, Dong-Hyun Kim, Young-Jun Park, Sun-Min Seo, Jun-Won Yun, Jun Won Park and 6 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Scientific reports, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

16 authors.

Ji-Hun LeeDepartment of Laboratory Animal Medicine, College of Veterinary Medicine, Konkuk University, Seoul, 05029, South Korea.
Eun-Seon YooDepartment of Laboratory Animal Medicine, College of Veterinary Medicine, Konkuk University, Seoul, 05029, South Korea.
Na-Won KimDepartment of Laboratory Animal Medicine, College of Veterinary Medicine, Konkuk University, Seoul, 05029, South Korea.
Won-Yong ShimDepartment of Laboratory Animal Medicine, College of Veterinary Medicine, Konkuk University, Seoul, 05029, South Korea.
Han-Bi JeongDepartment of Laboratory Animal Medicine, College of Veterinary Medicine, Konkuk University, Seoul, 05029, South Korea.
Dong-Hyun KimDepartment of Laboratory Animal Medicine, College of Veterinary Medicine, Konkuk University, Seoul, 05029, South Korea.
Young-Jun ParkDepartment of Laboratory Animal Medicine, College of Veterinary Medicine, Konkuk University, Seoul, 05029, South Korea.
Sun-Min SeoDepartment of Laboratory Animal Medicine, College of Veterinary Medicine, Konkuk University, Seoul, 05029, South Korea.
Jun-Won YunLaboratory of Veterinary Toxicology, College of Veterinary Medicine, Research Institute for Veterinary Science, Seoul National University, Seoul, 08826, South Korea.
Jun Won ParkDepartment of Laboratory Animal Medicine, College of Veterinary Medicine, Seoul National University, Seoul, 08826, South Korea.
Kang-Seuk ChoiLaboratory of Avian Diseases, College of Veterinary Medicine, Research Institute for Veterinary Science, Seoul National University, Seoul, 08826, South Korea.
Ho-Young LeeDepartment of Nuclear Medicine, Seoul National University Bundang Hospital, Seongnam, 13488, South Korea.
Jun-Young SeoDepartment of Biomedical Sciences, Graduate School of Medical Science, Brain Korea 21 Project, Yonsei University College of Medicine, Seoul, 03722, South Korea.
Ki Taek NamDepartment of Biomedical Sciences, Graduate School of Medical Science, Brain Korea 21 Project, Yonsei University College of Medicine, Seoul, 03722, South Korea.
Je Kyung SeongKorea Model Animal Priority Center, Seoul National University, Seoul, 08826, South Korea. snumouse@snu.ac.kr.
Yang-Kyu ChoiDepartment of Laboratory Animal Medicine, College of Veterinary Medicine, Konkuk University, Seoul, 05029, South Korea. yangkyuc@konkuk.ac.kr.

Funding

Bio & Medical Technology Development Program of the National Research Foundation (NRF), funded by the Korean government (MSIT) 2021M3H9A1030260Bio & Medical Technology Development Program of the National Research Foundation (NRF), funded by the Korean government (MSIT) 2021M3H9A1097269
6 · The paper itself

Abstract

Coronavirus disease-2019 (COVID-19), attributed to the severe acute respiratory syndrome-related coronavirus-2 (SARS-CoV-2), has posed global health challenges since it first emerged in 2019, and its impact continues to persist. The neurotropic nature of SARS-CoV-2 remains undisclosed, though researchers are proposing hypotheses on how the virus is transmitted to the central nervous system. One of the prevailing hypotheses is that SARS-CoV-2 travels through the olfactory nerve system via the olfactory epithelium (OE). Using a K18-human angiotensin converting-enzyme 2 (hACE2) transgenic mouse model with impaired olfactory sensory neurons (OSNs) induced by zinc sulfate, we examined the role of the olfactory nerve in the brain invasion by SARS-CoV-2. Mice lacking OSNs exhibited reduced levels of viral transmission to the brain, leading to significantly improved outcomes following SARS-CoV-2 infection. Moreover, a positive correlation was observed between viral persistence in the OE and brain infection. These results indicate that early inhibition of the olfactory nerve pathway effectively prevents viral invasion of the brain in K18-hACE2 mice. Our study underscores the significance of the olfactory nerve pathway in the transmission of SARS-CoV-2 to the brain.

Indexed as

Angiotensin-Converting Enzyme 2BrainCOVID-19Disease Models, AnimalMice, TransgenicOlfactory Receptor NeuronsSARS-CoV-2Zinc SulfateAnimalsHumansMiceOlfactory MucosaOlfactory NerveACE2 protein, humanAngiotensin-Converting Enzyme 2Zinc SulfateBrainK18-hACE2Olfactory epitheliumOlfactory sensory neuronsSARS-CoV-2Zinc sulfate

Identifiers

PMID39537718
PMCPMC11561319

What OpenQuestion holds

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LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.