Evidence map›Paper›PMID 39537343›Full record

ArticleAnalytical chemistry2024

Synthesis and Characterization of a Novel Intrinsically Fluorescent Analog of Cholesterol with Improved Photophysical Properties.

Max Lehmann, Senjuti Halder, Peter Reinholdt, Mohammad Bashawat, Holger A Scheidt, Jenny Leopold, Jürgen Schiller, Duccio di Prima, Vibeke Akkerman, Maria Szomek and 5 more

Abstract read
In one paragraph

Article in Analytical chemistry, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Review of methods for the determination of cellular cholesterol content: principles, advantages, limitations, applications, and perspectives.Analytical sciences : the international journal of the Japan Society for Analytical Chemistry · 2025
    Review
  2. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

15 authors.

Max LehmannDepartment of Chemistry, University of Potsdam, Karl-Liebknecht Str. 24-25, Potsdam 14476, Germany.
Senjuti HalderDepartment of Biochemistry and Molecular Biology, University of Southern Denmark, Odense M DK-5230, Denmark.
Peter ReinholdtDepartment of Physics, Chemistry and Pharmacy, University of Southern Denmark, Odense M DK-5230, Denmark.ORCID 0000-0003-2406-700X
Mohammad BashawatDepartment of Biology, Humboldt University Berlin, Invalidenstr. 42, Berlin 10115, Germany.
Holger A ScheidtInstitute for Medical Physics and Biophysics, Leipzig University, Härtelstr. 16-18, Leipzig 04107, Germany.ORCID 0000-0001-9935-8229
Jenny LeopoldInstitute for Medical Physics and Biophysics, Leipzig University, Härtelstr. 16-18, Leipzig 04107, Germany.
Jürgen SchillerInstitute for Medical Physics and Biophysics, Leipzig University, Härtelstr. 16-18, Leipzig 04107, Germany.ORCID 0000-0002-4328-8652
Duccio di PrimaDepartment of Physics, Chemistry and Pharmacy, University of Southern Denmark, Odense M DK-5230, Denmark.
Vibeke AkkermanDepartment of Biochemistry and Molecular Biology, University of Southern Denmark, Odense M DK-5230, Denmark.
Maria SzomekDepartment of Biochemistry and Molecular Biology, University of Southern Denmark, Odense M DK-5230, Denmark.
Line LauritsenDepartment of Biochemistry and Molecular Biology, University of Southern Denmark, Odense M DK-5230, Denmark.
Jacob KongstedDepartment of Physics, Chemistry and Pharmacy, University of Southern Denmark, Odense M DK-5230, Denmark.ORCID 0000-0002-7725-2164
Peter MüllerDepartment of Biology, Humboldt University Berlin, Invalidenstr. 42, Berlin 10115, Germany.
Pablo WessigDepartment of Chemistry, University of Potsdam, Karl-Liebknecht Str. 24-25, Potsdam 14476, Germany.
Daniel WüstnerDepartment of Biochemistry and Molecular Biology, University of Southern Denmark, Odense M DK-5230, Denmark.ORCID 0000-0003-4995-9709

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Live-cell imaging of cholesterol trafficking depends on suitable cholesterol analogs. However, existing fluorescent analogs of cholesterol either show very different physicochemical properties compared to cholesterol or demand excitation in the ultraviolet spectral region. We present a strategy to synthesize two novel intrinsically fluorescent sterol probes with a close resemblance of cholesterol. The analogs contain four conjugated double bonds in the ring system and either a keto group (probe

Indexed as

CholesterolFluorescent DyesMolecular Dynamics SimulationHumansCholesterolFluorescent Dyes

Identifiers

PMID39537343
PMCPMC11603404

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.