Evidence map›Paper›PMID 39536984›Full record

ArticleGeneral and comparative endocrinology2025

Spatial and quantitative gene expression analysis of SREB receptors in the gonads of green-spotted pufferfish (Dichotomyctere nigroviridis).

Timothy S Breton, Maria Eduarda Oliveira, Truly Chillemi, William Harriman, Joanna Korasadowicz, Eme Saverese, Emma Bourget, Casey A Murray, Christopher J Martyniuk, Matthew A DiMaggio

Abstract read
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In one paragraph

Article in General and comparative endocrinology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Timothy S BretonBiology Department, University of Maine at Farmington, Farmington, ME 04938, USA. Electronic address: timothy.breton@maine.edu.
Maria Eduarda OliveiraBiology Department, University of Maine at Farmington, Farmington, ME 04938, USA.
Truly ChillemiBiology Department, University of Maine at Farmington, Farmington, ME 04938, USA.
William HarrimanBiology Department, University of Maine at Farmington, Farmington, ME 04938, USA.
Joanna KorasadowiczBiology Department, University of Maine at Farmington, Farmington, ME 04938, USA.
Eme SavereseBiology Department, University of Maine at Farmington, Farmington, ME 04938, USA.
Emma BourgetBiology Department, University of Maine at Farmington, Farmington, ME 04938, USA.
Casey A MurrayTropical Aquaculture Laboratory, Program in Fisheries and Aquatic Sciences, School of Forest, Fisheries, and Geomatics Sciences, Institute of Food and Agricultural Sciences, University of Florida, Ruskin, FL 33570, USA.
Christopher J MartyniukCenter for Environmental and Human Toxicology, Department of Physiological Sciences, College of Veterinary Medicine, University of Florida, Gainesville, FL 32611, USA.
Matthew A DiMaggioTropical Aquaculture Laboratory, Program in Fisheries and Aquatic Sciences, School of Forest, Fisheries, and Geomatics Sciences, Institute of Food and Agricultural Sciences, University of Florida, Ruskin, FL 33570, USA.

Funding

The Maine Biomedical Research Network (INBRE)P20GM103423 · NIGMS · MOUNT DESERT ISLAND BIOLOGICAL LAB · PI Dustin Lynn Updike · 2012 to 2026
$60.0M
NIGMS NIH HHS P20 GM103423
6 · The paper itself

Abstract

Super-conserved Receptors Expressed in Brain (SREB) are a highly conserved family of orphan G protein-coupled receptors that consist of three members in most vertebrates: SREB1 (GPR27), SREB2 (GPR85), and SREB3 (GPR173). Each receptor is associated with diverse physiological processes and expressed in both ovaries and testes, but reproductive functions are only beginning to be understood. In addition, some fishes gained a novel fourth gene, SREB3B, which may have unique functions. The purpose of this study was to conduct a spatial and quantitative analysis of SREBs in the gonads of pufferfish (Dichotomyctere nigroviridis), which expresses all four genes. Multiplex RNAscope and absolute qPCR were used to assess gene expression patterns in both ovaries and testes. Expression was detected in early ovaries and dominated by sreb1 (approximately 2500 copies/ng RNA vs. 300 or less for others), with notable expression of all receptors in primary oocytes, granulosa cells, and small numbers of extra-follicular cells. Within primary oocytes, sreb1 and sreb3b exhibited diffuse patterns that may indicate early functions, while sreb2 and sreb3a were granular and may reflect stored mRNA. Early testicular development was dominated by sreb1 and sreb2 (∼5000 copies/ng RNA) in spermatogonia. These patterns were somewhat reduced in late testes (∼1000-2600 copies/ng RNA), but sreb3b exhibited a novel spatial pattern (∼380 copies/ng RNA) within spermatogenic cysts. These results highlight diverse roles for the SREB family, and sreb3b is hypothesized to have unique roles in fish reproduction.

Indexed as

OvaryTetraodontiformesAnimalsFemaleFish ProteinsGonadsMaleReceptors, G-Protein-CoupledTestisFish ProteinsReceptors, G-Protein-CoupledGPR173GPR27GPR85OocyteReproductionSpermatogoniaSREB

Identifiers

PMID39536984
PMCPMC11646178

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