Evidence map›Paper›PMID 39535711›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2025

Measurement of rRNA Synthesis and Degradation Rates by

María J García-Marcelo, Gaurav Singh, Sebastián Chávez, José E Pérez-Ortín

Abstract read
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In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

María J García-MarceloInstituto de Biotecnología y Biomedicina (BIOTECMED), Facultad de Biológicas, Universitat de València, Burjassot, Spain.
Gaurav SinghInstituto de Biotecnología y Biomedicina (BIOTECMED), Facultad de Biológicas, Universitat de València, Burjassot, Spain.
Sebastián ChávezInstituto de Biomedicina de Sevilla, Universidad de Sevilla-CSIC-Hospital Universitario V. del Rocío, Seville, Spain.
José E Pérez-OrtínInstituto de Biotecnología y Biomedicina (BIOTECMED), Facultad de Biológicas, Universitat de València, Burjassot, Spain. Jose.e.perez@uv.es.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

In order to measure the actual synthesis and degradation rates (SR, DR) for rRNA in yeast, we developed a method based on the pulse labeling and quantification of newly synthesized large rRNA molecules by a known mass of cells. The SR is calculated as the ratio of new rRNA molecules (synthesized after a short [5,6-

Indexed as

RNA, RibosomalSaccharomyces cerevisiaeUracilBlotting, NorthernIsotope LabelingRNA, FungalRNA StabilityYeastsRNA, FungalRNA, RibosomalUracilDegradation rateRNA polymerase IrRNASaccharomyces cerevisiaeSynthesis rateTranscription rateYeast

Identifiers

PMID39535711

What OpenQuestion holds

Textmetadata
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.