ArticleMethods in molecular biology (Clifton, N.J.)2025
Assessing Deadenylation Activity by Polyacrylamide Gel Electrophoresis Using a Fluorescent RNA Substrate.
Article in Methods in molecular biology (Clifton, N.J.), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
0 citing papers in PubMed.
No citing paper in PubMed yet.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The poly(A) tail is a dynamic structure at the 3'- end of the majority of RNA polymerase II transcripts. It is a critical feature, particularly for mRNAs, as the length of the poly(A) tail regulates their translational efficiency and lifespan. The shortening of the tail is catalyzed by deadenylases that trim and finally remove it, triggering mRNA degradation. Conventional assays to evaluate and measure deadenylase activity rely on radiolabeling of the substrate while colorimetric alternatives lack sensitivity. In this chapter, we describe a poly(A)-shortening assay using a 5'-Cy3-labeled RNA substrate bearing a 3'-oligo(A) sequence. The reaction products representing substrates with shortened tails are analyzed by denaturing acrylamide electrophoresis and are visualized using a gel documentation system. This assay is efficient, it requires standard lab equipment, including a fluorescence-detecting gel documentation system, and presents a quantifiable and safer alternative to radioactivity-based methods for evaluating deadenylase activity.
Indexed as
Identifiers
39535704What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.