Evidence map›Paper›PMID 39535701›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2025

Northern Blotting: Protocols for Radioactive and Nonradioactive Detection of RNA.

Maxime Wery, Dominika Foretek, Sara Andjus, Perrine Verdys, Antonin Morillon

Abstract read
PubMed Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Maxime WeryncRNA, Epigenetic and Genome Fluidity, CNRS UMR3244, Sorbonne Université, PSL University, Institut Curie, Paris, France, Paris Cedex 05, France. maxime.wery@curie.fr.
Dominika ForetekncRNA, Epigenetic and Genome Fluidity, CNRS UMR3244, Sorbonne Université, PSL University, Institut Curie, Paris, France, Paris Cedex 05, France.
Sara AndjusncRNA, Epigenetic and Genome Fluidity, CNRS UMR3244, Sorbonne Université, PSL University, Institut Curie, Paris, France, Paris Cedex 05, France.
Perrine VerdysncRNA, Epigenetic and Genome Fluidity, CNRS UMR3244, Sorbonne Université, PSL University, Institut Curie, Paris, France, Paris Cedex 05, France.
Antonin MorillonncRNA, Epigenetic and Genome Fluidity, CNRS UMR3244, Sorbonne Université, PSL University, Institut Curie, Paris, France, Paris Cedex 05, France. antonin.morillon@curie.fr.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Northern blotting is a common technique in RNA biology, allowing to detect and quantify RNAs of interest following separation by gel electrophoresis, transfer to a membrane, and hybridization of specific anti-complementary labelled probes. In this chapter, we describe our protocol for efficient RNA extraction from yeast, separation on agarose gel, and capillary transfer to a membrane. We provide two different methods for strand-specific detection of several types of RNAs using oligonucleotide probes, the first using radioactive

Indexed as

Blotting, NorthernRNADigoxigeninElectrophoresis, Agar GelNucleic Acid HybridizationOligonucleotide ProbesPhosphorus RadioisotopesRNA, FungalDigoxigeninOligonucleotide ProbesPhosphorus RadioisotopesRNARNA, Fungal32P-labelled oligonucleotideDigoxigeninLong noncoding RNAmRNA decayNonradioactive Northern blottingNorthern blottingTotal RNA extractionXrn1-sensitive lncRNAYeast

Identifiers

PMID39535701

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.