A Fluorogenic Sensor via Catalytic Hairpin Assembly for Precise Live-Cell Imaging of mRNA.

Su-Jing Zhao, Zhenkun Wu, Jian-Hui Jiang

Abstract read

In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it

0cells of the map it votes in

0citing papers in PubMed

–field-weighted citation impact

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

Su-Jing ZhaoState Key Laboratory of Chemo/Bio-Sensing and Chemometrics, College of Chemistry and Chemical Engineering, College of Biomedical Sciences, Hunan University, Changsha, China.

Zhenkun WuState Key Laboratory of Chemo/Bio-Sensing and Chemometrics, College of Chemistry and Chemical Engineering, College of Biomedical Sciences, Hunan University, Changsha, China. tomwu@hnu.edu.cn.

Jian-Hui JiangState Key Laboratory of Chemo/Bio-Sensing and Chemometrics, College of Chemistry and Chemical Engineering, College of Biomedical Sciences, Hunan University, Changsha, China.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Accurate evaluation of the tumor-related mRNA expression levels provides important information for cancer diagnosis and therapy. We recently reported a fluorogenic sensor by coupling tetrazine-mediated bioorthogonal reaction with catalytic hairpin assembly for precise imaging of GalNac-T mRNA in live cells. Fluorescence signals are specifically generated by target mRNA triggered spatial localization of bioorthogonal chemicals. The proposed fluorogenic sensor exhibits low detection limit with signal amplification process. Importantly, the method can avoid false-positive results in biological environment because of the "click to release" feature of bioorthogonal reaction. Our method may provide a useful tool for precise imaging of intracellular mRNA with low expression levels.

Indexed as

Fluorescent DyesRNA, MessengerBiosensing TechniquesHumansInverted Repeat SequencesMolecular ImagingOptical ImagingFluorescent DyesRNA, MessengerBioorthogonal reactionBiosensorCatalytic hairpin assemblymRNA imaging

Identifiers

PMID39535648

DOI10.1021/acs.analchem.1c05509

What OpenQuestion holds

Textmetadata

Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.