Evidence map›Paper›PMID 39535422›Full record

ArticleAdvanced healthcare materials2025

Hydrogel Elastic Energy: A Stressor Triggering an Adaptive Stress-Mediated Cell Response.

Sara Lipari, Pasquale Sacco, Michela Cok, Francesca Scognamiglio, Maurizio Romano, Francesco Brun, Piero Giulio Giulianini, Eleonora Marsich, Finn L Aachmann, Ivan Donati

Abstract read
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In one paragraph

Article in Advanced healthcare materials, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Article
  2. Review
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Sara LipariDepartment of Life Sciences, University of Trieste, Via L. Giorgieri 5, Trieste, 34127, Italy.ORCID 0009-0008-4058-7922
Pasquale SaccoDepartment of Life Sciences, University of Trieste, Via L. Giorgieri 5, Trieste, 34127, Italy.ORCID 0000-0002-4483-5099
Michela CokDepartment of Life Sciences, University of Trieste, Via L. Giorgieri 5, Trieste, 34127, Italy.
Francesca ScognamiglioDepartment of Life Sciences, University of Trieste, Via L. Giorgieri 5, Trieste, 34127, Italy.
Maurizio RomanoDepartment of Life Sciences, University of Trieste, Via L. Giorgieri 5, Trieste, 34127, Italy.
Francesco BrunDepartment of Engineering and Architecture, University of Trieste, Via A. Valerio 6/1, Trieste, 34127, Italy.
Piero Giulio GiulianiniDepartment of Life Sciences, University of Trieste, Via L. Giorgieri 5, Trieste, 34127, Italy.
Eleonora MarsichDepartment of Medicine, Surgery and Health Sciences, University of Trieste, Piazza dell'Ospitale 1, Trieste, 34129, Italy.
Finn L AachmannDepartment of Biotechnology and Food Science, Norwegian Biopolymer Laboratory (NOBIPOL), NTNU Norwegian University of Science and Technology, Sem Sælands vei 6/8, Trondheim, 7491, Norway.
Ivan DonatiDepartment of Life Sciences, University of Trieste, Via L. Giorgieri 5, Trieste, 34127, Italy.

Funding

European Union-Next Generation EU Decreto Ministeriale n. 352European Union's Horizon 2020 research and innovation program 817992Ministero dell'Istruzione, dell'Università e della Ricerca Decreto Direttoriale n. 241Norwegian Research Council 311958
6 · The paper itself

Abstract

The crosstalk between the cells and the extracellular matrix (ECM) is bidirectional and consists of a pushing/pulling stretch exerted by the cells and a mechanical resistance counteracted by the surrounding microenvironment. It is widely recognized that the stiffness of the ECM, its viscoelasticity, and its overall deformation are the most important traits influencing the response of the cells. Here these three parameters are combined into a concept of elastic energy, which in biological terms represents the mechanical feedback that cells perceive when the ECM is deformed. It is shown that elastic energy is a stress factor that influences the response of cells in three-dimensional (3D) cultures. Strikingly, the higher the elastic energy of the matrix and thus the mechanical feedback, the higher the stress state of the cells, which correlates with the formation of G3BP-mediated stress granules. This condition is associated with an increase in alkaline phosphatase (ALP) activity but a decrease in gene expression and is mediated by the nuclear translocation of Yes-associated protein (YAP). This work supports the importance of considering the elastic energy as mechano-controller in regulating cellular stress state in 3D cultures.

Indexed as

HydrogelsStress, MechanicalAdaptor Proteins, Signal TransducingAlkaline PhosphataseCell Culture Techniques, Three DimensionalElasticityExtracellular MatrixHumansYAP-Signaling ProteinsAdaptor Proteins, Signal TransducingAlkaline PhosphataseHydrogelsYAP1 protein, humanYAP-Signaling Proteinscellular stress stateextracellular matrix mimichydrogelmechanical feedbackmechanosensing

Identifiers

PMID39535422
PMCPMC11730662

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.