ArticleFrontiers in cellular and infection microbiology2024
Bronchoalveolar lavage fluid and lung biopsy tissue metagenomic next-generation sequencing in the diagnosis of pulmonary cryptococcosis.
Article in Frontiers in cellular and infection microbiology, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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12 citing papers in PubMed.
- Article
- Clinical spectrum, immune status, and prognostic factors of cryptococcosis: insights from a large, multi-center, ambispective cohort study in southeastern China.Infectious diseases of poverty · 2026Article
- Exhaled breath condensate metabolomics reveals inflammation-associated metabolic alterations and metabolites with possible relevance to virulence-related processes in pulmonary cryptococcosis.Frontiers in cellular and infection microbiology · 2026Article
- The diagnostic value of fine-needle aspiration cytology in the early diagnosis of pulmonary cryptococcosis.Revista do Instituto de Medicina Tropical de Sao Paulo · 2026Article
- Metagenomic next-generation sequencing: new horizons in microbiology.Frontiers in cellular and infection microbiology · 2026Review
- Pulmonary sarcoidosis complicated with pulmonary cryptococcosis: a case report.Frontiers in medicine · 2026Article
- Late-onset fungal infection of the bronchial stump post-lung resection: a report of two rare cases ofAME case reports · 2026Article
- Evaluating the diagnostic efficacy of metagenomic next-generation sequencing in bronchoalveolar lavage fluid for pulmonary cryptococcosis with different clinical and imaging characteristics.BMC infectious diseases · 2025Article
- Overcoming the Diagnostic Challenges in Cutaneous Cryptococcosis Caused by Cryptococcus neoformans: A Case Study Utilizing Metagenomic Next-Generation Sequencing.Mycopathologia · 2025Article
- Diagnostic value of combined detection of cryptococcal antigen in serum and lung puncture fluid using lateral flow assay for diagnosing pulmonary cryptococcosis: a single-center prospective study.Frontiers in microbiology · 2025Article
- Metagenomic next-generation sequencing facilitates precision treatment and prognostic improvement in pulmonary cryptococcosis.Frontiers in cellular and infection microbiology · 2025Article
- Targeted next-generation sequencing using bronchoalveolar lavage fluid samples for diagnosing pulmonary infections: a single-center retrospective study.Frontiers in microbiology · 2025Article
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7 authors.
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Abstract
Objective: To evaluate the diagnostic value of metagenomic next-generation sequencing (mNGS) in pulmonary cryptococcosis (PC) using bronchoalveolar lavage fluid (BALF) and lung biopsy tissue specimens. Methods: In this retrospective study, 321 patients diagnosed with lower respiratory tract diseases who underwent mNGS using BALF and LBT samples, between January 2021 and December 2023 were included. Individuals were classified into PC and non-PC groups according to the diagnostic criteria for PC, and conventional fungal cultures were performed. A serum/BALF cryptococcal antigen (CrAg) test was performed in some patients with PC. The diagnostic efficiencies of three methods for PC (mNGS, conventional culture, and CrAg) were compared. Additionally, two mNGS methods were used in this study: original mNGS (OmNGS, testing time from January 2021 to December 2022) and modified mNGS (MmNGS, testing time from January to December 2023). The diagnostic efficiency of the two mNGS methods on PC was simultaneously compared. Results: Among the 321 patients, 23 (7.2%) had PC and 298 (92.8%) did not. Compared with the composite reference standard for PC diagnosis, the sensitivity, specificity, and accuracy of mNGS for PC were 78.3% (95% confidence interval [CI], 55.8%-91.7%), 98.7% (95% CI, 96.4%-99.6%), and 97.2% (95% CI, 94.7%-98.7%), respectively. The sensitivity of mNGS was similar to that of CrAg (80.0%, 12/15) ( Conclusion: The sensitivity of mNGS is better than that of conventional culture. The combination of mNGS and CrAg improves the testing sensitivity of
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