ReviewCommunications chemistry2024
Liquid-liquid crystalline phase separation of spider silk proteins.
Review in Communications chemistry, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
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Who cites it
17 citing papers in PubMed.
- Biomolecular coacervation-mediated materials: Phase states, phase transitions, and biomedical applications.Bioactive materials · 2027Review
- Coacervates as bio-inspired intermediates for materials processing.Chemical communications (Cambridge, England) · 2026Review
- Diverging Liquid-Liquid Phase Separation Behavior of Different Recombinant Major Ampullate Spidroins.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- Liquid-liquid phase separation enables chromatography-free purification and high-performance spidroin-amyloid hybrid silk fibers.Proceedings of the National Academy of Sciences of the United States of America · 2026Article
- Advances in FRET-based Applications Within Biomimicking Molecular Self-assemblies.Journal of fluorescence · 2026Review
- Self-assembling proteins compose the chemically resistant shell biomaterial of planktonic tintinnid ciliates.Nature communications · 2026Article
- Engineering Design of Artificial Phase-Separating Proteins.Biotechnology journal · 2026Review
- Directed Functionalization of Recombinant Spider Silk Nonwoven Membranes with Antibodies Using Non-Canonical Amino Acids.Advanced materials (Deerfield Beach, Fla.) · 2026Article
- A Mini-Spidroin Forms High-Performance Artificial Spider Silk via Edge-Cysteine-Locked β-Sheet Assembly.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- Spider Silk Inspired Processing of Liquid Crystalline Complex Coacervates.ACS macro letters · 2026Article
- pH-triggered clustering regulates β-sheet activation in silk assembly.Communications chemistry · 2026Article
- Wrinkling-Based Patterning and Recombinant Spider Silk-Based Coating Technologies - Toward Novel Applications.Small (Weinheim an der Bergstrasse, Germany) · 2026Review
- Arg-Tyr cation-π interactions drive phase separation and β-sheet assembly in native spider dragline silk.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Remodeling and self-healing of individual amyloid tactoids via multiphoton absorption.Nature communications · 2025Article
- Nanostructured Protein Surfaces Inspired by Spider Silk.Advanced materials (Deerfield Beach, Fla.) · 2025Review
- Crosslinking, salt-induced aging, and secondary structure formation in Peptide-containing coacervates inspired by spider silk.Communications chemistry · 2025Article
- Native Mass Spectrometry Captures the Conformational Plasticity of Proteins with Low-Complexity Domains.JACS Au · 2025Article
Corrections and comments
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Liquid-liquid phase separation (LLPS) of proteins can be considered an intermediate solubility regime between disperse solutions and solid fibers. While LLPS has been described for several pathogenic amyloids, recent evidence suggests that it is similarly relevant for functional amyloids. Here, we review the evidence that links spider silk proteins (spidroins) and LLPS and its role in the spinning process. Major ampullate spidroins undergo LLPS mediated by stickers and spacers in their repeat regions. During spinning, the spidroins droplets shift from liquid to crystalline states. Shear force, altered ion composition, and pH changes cause micelle-like spidroin assemblies to form an increasingly ordered liquid-crystalline phase. Interactions between polyalanine regions in the repeat regions ultimately yield the characteristic β-crystalline structure of mature dragline silk fibers. Based on these findings, we hypothesize that liquid-liquid crystalline phase separation (LLCPS) can describe the molecular and macroscopic features of the phase transitions of major ampullate spidroins during spinning and speculate whether other silk types may use a similar mechanism to convert from liquid dope to solid fiber.
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.