ArticleScientific reports2024
Genetic remodeling of soil diazotrophs enables partial replacement of synthetic nitrogen fertilizer with biological nitrogen fixation in maize.
Article in Scientific reports, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed.
- Beyond Nitrogen Fixation: Multifunctional Roles of Common Bean in Organic Agroecosystems and Ecosystem-Oriented Breeding Strategies.Plants (Basel, Switzerland) · 2026Review
- Environmental Release of Genetically Intervened Microorganisms: Towards a New Narrative.Microbial biotechnology · 2026Review
- Engineered diazotrophs with host-inducible nitrogen supply systems: Transforming rice farming through innovative nitrogen biofertilizers.Journal of integrative plant biology · 2026Review
- Nitrogenase regulation inbioRxiv : the preprint server for biology · 2026Article
- Nitrogen Application Under Integrated Water-Fertilizer Management Regulates Rhizosphere Nitrogen Dynamics, Microbial Communities, and Maize Productivity.Microbial ecology · 2026Article
- Isolation of N-Fixing Bacteria from Warm-Season Pasture Grasses and the Evaluation of Nitrogen Effects on the Bacterial Communities Present inMicroorganisms · 2026Article
- Nitrogen-Controlled Host Gatekeeping: Regulatory Chokepoints Across Four Windows for Diazotroph Access.International journal of molecular sciences · 2026Article
- Dynamics of genetic circuits in Pseudomonas protegens.Cell systems · 2026Article
- Synthetic Biology of Plants and Microbes for Agriculture, Environment, and Future Applications.Chemical reviews · 2026Review
- Molecular mechanisms modulating beneficial plant root-microbe interactions: What's common?Plant communications · 2026Review
- Developments and food safety considerations concerning genetically engineered microbial crop protection agents and biostimulants.Journal of pesticide science · 2025Article
- Harnessing plant agriculture to mitigate climate change: A framework to evaluate synthetic biology (and other) interventions.Plant physiology · 2025Article
- Be a GEM: Biocontained, environmentally applied, genetically engineered microbes.Advanced drug delivery reviews · 2025Review
- Engineering Biology's Vital Role for Climate Mitigation.Engineering biologyArticle
Corrections and comments
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Authors and funding
30 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Increasing biological nitrogen (N) fixation (BNF) in maize production could reduce the environmental impacts of N fertilizer use, but reactive N in the rhizosphere of maize limits the BNF process. Using non-transgenic methods, we developed gene-edited strains of Klebsiella variicola (Kv137-2253) and Kosakonia sacchari (Ks6-5687) bacteria optimized for root-associated BNF and ammonium excretion in N-rich conditions. The aim of this research was to elucidate the mechanism of action of these strains. We present evidence from in vitro, in planta and field experiments that confirms that our genetic remodeling strategy derepresses BNF activity in N-rich systems and increases ammonium excretion by orders of magnitude above the respective wildtype strains. BNF is demonstrated in controlled environments by the transfer of labeled
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