Dynamic maternal synthesis and segregation of the germ plasm organizer, Bucky ball, in chicken oocytes and follicles.

Sabine Klein, Roland Dosch, Sven Reiche, Wilfried A Kues

Abstract read

In one paragraph

Article in Scientific reports, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it

0cells of the map it votes in

0citing papers in PubMed

–field-weighted citation impact

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Sabine KleinFriedrich-Loeffler-Institut, Institute of Farm Animal Genetics, Department of Biotechnology, Stem Cell Unit, Mariensee, Höltystr. 10, 31535, Neustadt, Germany. sabine.klein@fli.de.

Roland DoschInstitut Für Humangenetik, Department of Developmental Biochemistry, Georg-August-Universität Göttingen, 37077, Göttingen, Germany.

Sven ReicheDept. of Experimental Animal Facilities and Biorisk Management, Friedrich-Loeffler-Institut, Südufer 10, 17493, Greifswald - Insel Riems, Germany.

Wilfried A KuesFriedrich-Loeffler-Institut, Institute of Farm Animal Genetics, Department of Biotechnology, Stem Cell Unit, Mariensee, Höltystr. 10, 31535, Neustadt, Germany.

Funding

European Virus Archive GLOBAL (EVA-GLOBAL) project that has received funding from the European Union's Horizon 2020 research and innovation program 871029

6 · The paper itself

Abstract

Maternal germ plasm determines the germline in birds. Previously, we proposed the chicken-specific Bucky ball (cBuc) as a functional equivalent of the zebrafish germ plasm organizer. This study demonstrated the maternal cBuc synthesis, and verified a highly dynamic distribution of Bucky ball from oocyte nests to maturing follicles using specific antibodies. The dynamic re-localization of cBuc from the ovarian stroma to the granulosa cells, and the Balbiani structure of the oocyte was revealed. Following the accumulation of cBuc in the Balbiani body, an increased signal of chicken vasa homolog (CVH) in close contact to cBuc could be detected. Highest transcription of cBuc was recorded in follicles with diameters up to 500 µm. First RNA-interference experiments in an in-vivo follicle culture assay revealed inhibiting effects on cBuc in small follicles. These data demonstrate the maternal origin of cBuc, and underpin its role as germ plasm organizer.

Indexed as

ChickensOocytesOvarian FollicleAnimalsFemaleGerm CellsGranulosa CellsBalbiani bodyChickenFolliculogenesisGermline specificationGerm plasmOocytes

Identifiers

PMID39532932

DOI10.1038/331070a0

PMCPMC11557578

What OpenQuestion holds

Textmetadata

LicenceCC BY

Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.