Evidence map›Paper›PMID 39531058›Full record

ArticleApplied microbiology and biotechnology2024

Surface monitoring of L. monocytogenes by real-time fluorescence and colorimetric LAMP.

Maruxa Abalo, Alexandre Lamas, Carla Teixeira, Marta Prado, Alejandro Garrido-Maestu

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Applied microbiology and biotechnology, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Maruxa AbaloDepartment of Functional Biology, University of Santiago de Compostela, Santiago de Compostela, Spain.
Alexandre LamasFood Hygiene, Inspection and Control Laboratory (Lhica), Department of Analytical Chemistry, Nutrition and Bromatology, Veterinary School, Campus Terra, University of Santiago de Compostela, 27002, Lugo, Spain.
Carla TeixeiraFood Quality & Safety Research Group, International Iberian Nanotechnology Laboratory, Av. Mestre José Veiga S/N, 4715-330, Braga, Portugal.
Marta PradoFood Quality & Safety Research Group, International Iberian Nanotechnology Laboratory, Av. Mestre José Veiga S/N, 4715-330, Braga, Portugal.
Alejandro Garrido-MaestuFood Quality & Safety Research Group, International Iberian Nanotechnology Laboratory, Av. Mestre José Veiga S/N, 4715-330, Braga, Portugal. alejandro.garrido@inl.int.ORCID http://orcid.org/0000-0003-4587-8418

Funding

Agência Nacional de Inovação C644915155-00000024Fundação para a Ciência e a Tecnologia 2021.02810.CEECIND
6 · The paper itself

Abstract

Listeria monocytogenes is a major foodborne pathogen affecting developing, and developed countries. The analysis of food contact surfaces in food industries is key for better controlling this pathogen. The current study focused on the development, optimization, and evaluation of a rapid and simple method for the detection of L. monocytogenes on stainless steel surfaces, suitable for decentralized setups, taking advantage of Loop-mediated isothermal amplification (LAMP). This was accomplished using a general pre-enrichment broth (TSB), with a simple DNA extraction based on a chelating resin, and final isothermal amplification. Two different detection strategies were tested, real-time fluorescence and naked-eye colorimetric, which were evaluated after 5, 7, and 24 h of pre-enrichment. Regardless the detection chemistry selected, after 5-7 h of pre-enrichment, 10

Indexed as

ColorimetryFood MicrobiologyListeria monocytogenesNucleic Acid Amplification TechniquesDNA, BacterialFluorescenceMolecular Diagnostic TechniquesSensitivity and SpecificityStainless SteelDNA, BacterialStainless SteelListeria monocytogenesLoop-mediated isothermal amplificationNaked-eye detectionStainless steelSurface analysis

Identifiers

PMID39531058
PMCPMC11557679

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.