ArticleEmerging microbes & infections2024
Analytical and clinical evaluation of a duplex RT-qPCR assay for the detection and identification of o'nyong-nyong and chikungunya virus.
Article in Emerging microbes & infections, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
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Who cites it
7 citing papers in PubMed.
- Modulating Chikungunya and Mayaro virus-induced disease severity in mice using low concentrations of anti-IFNAR1 antibodies.Emerging microbes & infections · 2026Article
- A rapid multiplex platform for simultaneous detection of chikungunya virus, dengue virus, and dengue serotyping based on isothermal amplification and lateral flow dipsticks.Infectious diseases of poverty · 2026Article
- A brief review of chikungunya fever: From molecular virology to countermeasures.Infectious medicine · 2026Review
- Application of CRISPR-Cas12a/Cas13a dual enzyme cleavage technology in the differential diagnosis of Chikungunya virus and Dengue virus.Journal of biological engineering · 2026Article
- Arbovirus diagnostics: from conventional methods to emerging technologies-a chikungunya virus-centric review.Frontiers in microbiology · 2026Review
- Development and evaluation of a duplex RT-qPCR assay for the detection and identification of Mayaro and chikungunya viruses.Journal of clinical microbiology · 2025Article
- Development of a capillary-modified naked-eye visual loop-mediated isothermal amplification method for the rapid detection of mpox virus and chikungunya virus.Frontiers in microbiology · 2025Article
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The mosquito-borne alphavirus o'nyong-nyong virus (ONNV) has proven its potential to cause major human outbreaks. On the African continent, ONNV causes unspecific febrile illness and co-circulates with the close relative chikungunya virus (CHIKV). The true scale of ONNV burden is poorly understood in Africa, because of the scarce availability of molecular in-house and commercial assays, strong cross-reactivity between ONNV and CHIKV in serological assays and a lack of surveillance. We designed a new RT-qPCR assay targeting the E1 gene for the detection of ONNV that can be used in monoplex or in duplex format, combined with a previously published CHIKV monoplex assay targeting the nsp1. The lower limit of detection with 95% positivity rate was determined to be <10 RNA copies/µL in monoplex and duplex format for both ONNV and CHIKV. Both monoplex assays and the duplex assay proved to be linear within the tested range of approximately 10
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