Evidence map›Paper›PMID 39528669›Full record

ArticleCommunications engineering2024

Three-dimensional random-access confocal microscopy with 3D remote focusing system.

Haoyang Li, Quan Lu, Zhong Wang, Wenbo Zhang, Yu Wu, Yandong Sun, Yue Hu, Lehui Xiao, Dongping Zhong, Suhui Deng and 1 more

Abstract read
In one paragraph

Article in Communications engineering, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

Haoyang Li *Institute of Systems and Physical Biology, Shenzhen Bay Laboratory, Shenzhen, China.ORCID http://orcid.org/0000-0002-0494-299X
Quan Lu *Institute of Systems and Physical Biology, Shenzhen Bay Laboratory, Shenzhen, China.
Zhong Wang *Institute of Systems and Physical Biology, Shenzhen Bay Laboratory, Shenzhen, China.
Wenbo ZhangInstitute of Systems and Physical Biology, Shenzhen Bay Laboratory, Shenzhen, China.
Yu WuInstitute of Systems and Physical Biology, Shenzhen Bay Laboratory, Shenzhen, China.
Yandong SunInstitute of Systems and Physical Biology, Shenzhen Bay Laboratory, Shenzhen, China.
Yue HuSchool of Electronics and Information Engineering, Harbin Institute of Technology, Harbin, China.
Lehui XiaoCollege of Chemistry and Chemical Engineering, Central South University, Changsha, China.
Dongping ZhongCenter for Ultrafast Science and Technology, School of Chemistry and Chemical Engineering, Shanghai Jiao Tong University, Shanghai, China. dongping@sjtu.edu.cn.ORCID http://orcid.org/0000-0001-9381-8992
Suhui DengSchool of Information Engineering, Nanchang University, Nanchang, China. shdeng@ncu.edu.cn.ORCID http://orcid.org/0000-0003-3527-6093
Shangguo HouInstitute of Systems and Physical Biology, Shenzhen Bay Laboratory, Shenzhen, China. shangguo.hou@szbl.ac.cn.ORCID http://orcid.org/0000-0001-6394-6467

Funding

National Natural Science Foundation of China (National Science Foundation of China) 22204106National Natural Science Foundation of China (National Science Foundation of China) 62065012
6 · The paper itself

Abstract

Understanding biological activities in cells or deep tissues requires high-speed three-dimensional (3D) imaging. Substantial progress has been made with the emergence of 3D random-access microscopy. However, current solutions for fast 3D random-access imaging remain complex and costly. Herein we propose a simple, cost-effective, and fast 3D random-access confocal microscopy with remote focusing system. Our system shows isotropic response times across the x, y, and z axes, with a 34-fold improvement in axial response time over traditional piezo stages. We demonstrate its volumetric imaging performance with fluorescent particles and live cells. Furthermore, we validate the 3D random-access imaging capability of this system by continuously monitoring the signals in three different planes, showing a refresh rate of 500 Hz on two different positions in 3D. The simplicity, versatility, and affordability of our system promise widespread applications in research and industry.

Identifiers

PMID39528669
PMCPMC11555065

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.