ArticleScientific reports2024
Expression of key cytokines in dog macrophages infected by Leishmania tarentolae opening new avenues for the protection against Leishmania infantum.
Article in Scientific reports, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
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Who cites it
7 citing papers in PubMed.
- Performance assessment of a duplex quantitative PCR assay for detectingMicrobiology spectrum · 2026Article
- Leishmania infantum in red foxes (Vulpes vulpes): from clinical findings to cytokine expression.Parasites & vectors · 2026Article
- Cytokine gene expression in feline leishmaniasis: why cats might be less clinically affected than dogs.Parasites & vectors · 2026Article
- Epidemiological dynamics ofMicrobial genomics · 2025Article
- The effects of leukocyteBMC veterinary research · 2025Article
- Leishmania (Leishmania) infantum and Leishmania (Sauroleishmania) tarentolae in outdoor cats and report of infection in feline-derived peripheral blood mononuclear cells.Parasites & vectors · 2025Article
- Genetic Regulation of Immune Response in Dogs.Genes · 2025Review
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Authors and funding
8 authors.
Funding
Abstract
The detection of Leishmania tarentolae in sympatric areas where Leishmania infantum is endemic raised questions regarding the protective effect exerted in dogs by L. tarentolae when in coinfection. This study aimed monitoring the in vitro gene expression of pro- (IFN- γ; TNF-α; IL-12) and anti-inflammatory (IL-4; IL-6; IL-10) cytokines in primary canine macrophages infected by L. tarentolae and L. infantum in single and in co-infections. Macrophages differentiated from dog blood mononuclear cells were infected with the L. tarentolae field-isolated (RI-325) and laboratory (LEM-124) strains, with L. infantum laboratory strain (IPT1), or both. Infection and the number of amastigotes per infected cell were evaluated microscopically by counting a total of 200 cells between 4 and 96 h. Cytokine gene expression was analyzed by real-time PCR from infected macrophages mRNA. Single infections presented higher expression of the cytokines IL-4 and IL-6, and lower of IL-12. Co-infections induced a lower gene expression of IL-4 and IL-6, and a higher gene expression of IL-12, correlating with the low amastigote burden despite the slight increase of infected cells. Data highlight the potential protective effect of L. tarentolae against L. infantum in co-infection by the reduced anti-inflammatory and increased pro-inflammatory cytokines gene expression, opening new perspectives for a canine vaccine development exploiting the non-pathogenic L. tarentolae.
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