Evidence map›Paper›PMID 39528415›Full record

ArticleAnalytical chemistry2024

Development of an Automated, Ultra-Rapid Bottom-Up Proteomics Workflow Utilizing Alginate-Based Hydrogels.

Maor Arad, Connor Frey, Ronald Balagtas, Rhien Hare, Kenneth Ku, Dario Jereb, Zach Nestman, Anoop Sidhu, Yuming Shi, Osei Fordwour and 3 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Analytical chemistry, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. StageTip: a little giant unveiling the potential of mass spectrometry-based proteomics.Analytical sciences : the international journal of the Japan Society for Analytical Chemistry · 2025
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors.

Maor AradDepartment of Biochemistry and Molecular Biology, Michael Smith Laboratories, University of British Columbia, Vancouver, BC V6T 1Z4, Canada.ORCID 0000-0003-4618-9868
Connor FreyDepartment of Chemistry, University of the Fraser Valley, 33844 King Road, Abbotsford, BC V2S 7M7, Canada.ORCID 0000-0002-4080-3030
Ronald BalagtasDepartment of Chemistry, University of the Fraser Valley, 33844 King Road, Abbotsford, BC V2S 7M7, Canada.ORCID 0009-0009-7728-3751
Rhien HareDepartment of Chemistry, University of the Fraser Valley, 33844 King Road, Abbotsford, BC V2S 7M7, Canada.
Kenneth KuDepartment of Chemistry, University of the Fraser Valley, 33844 King Road, Abbotsford, BC V2S 7M7, Canada.ORCID 0000-0002-8905-5432
Dario JerebDepartment of Chemistry, University of the Fraser Valley, 33844 King Road, Abbotsford, BC V2S 7M7, Canada.
Zach NestmanDepartment of Chemistry, University of the Fraser Valley, 33844 King Road, Abbotsford, BC V2S 7M7, Canada.
Anoop SidhuDepartment of Chemistry, University of the Fraser Valley, 33844 King Road, Abbotsford, BC V2S 7M7, Canada.
Yuming ShiDepartment of Biochemistry and Molecular Biology, Michael Smith Laboratories, University of British Columbia, Vancouver, BC V6T 1Z4, Canada.
Osei FordwourDepartment of Biochemistry and Molecular Biology, Michael Smith Laboratories, University of British Columbia, Vancouver, BC V6T 1Z4, Canada.
Kyung-Mee MoonDepartment of Biochemistry and Molecular Biology, Michael Smith Laboratories, University of British Columbia, Vancouver, BC V6T 1Z4, Canada.
Leonard J FosterDepartment of Biochemistry and Molecular Biology, Michael Smith Laboratories, University of British Columbia, Vancouver, BC V6T 1Z4, Canada.ORCID 0000-0001-8551-4817
Golfam GhafourifarDepartment of Chemistry, University of the Fraser Valley, 33844 King Road, Abbotsford, BC V2S 7M7, Canada.ORCID 0000-0001-8024-1792

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

A new approach to sample preparation and enzymatic digestion in bottom-up proteomics has been developed using alginate-based hydrogel entrapment of enzymes. This hydrogel facilitates rapid and room-temperature digestions with multienzyme capabilities. Three methodologies were tested: within microcentrifuge tubes,

Indexed as

AlginatesHydrogelsProteomicsAutomationGlucuronic AcidHexuronic AcidsPepsin ATrypsinWorkflowAlginatesGlucuronic AcidHexuronic AcidsHydrogelsPepsin ATrypsin

Identifiers

PMID39528415
PMCPMC11603402

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.