Evidence map›Paper›PMID 39527553›Full record

ArticlePloS one2024

Extended-spectrum beta-lactamase-producing Enterobacterales in human health: Experience from the tricycle project, Ghana.

Noah Obeng-Nkrumah, Appiah Korang-Labi, Paul Kwao, Beverly Egyir, Benjamin D Nuertey, George Hedidor, Gifty Boateng, Kwaku Asah-Opoku, Thomas Dankwah, Esther Okine and 1 more

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Article in PloS one, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
7citing papers in PubMed, 1 pooled it
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

7 citing papers in PubMed, 1 synthesis or guideline pooled it.

  1. Pooled it
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  3. Article
  4. Article
  5. Article
  6. Article
  7. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

Noah Obeng-NkrumahDepartment of Medical Laboratory Sciences, School of Biomedical and Allied Health Sciences, College of Health Sciences, University of Ghana, Accra, Ghana.
Appiah Korang-LabiDepartment of Medical Microbiology, University of Ghana Medical School, College of Health Sciences, University of Ghana, Accra, Ghana.
Paul KwaoDepartment of Medical Laboratory Sciences, School of Biomedical and Allied Health Sciences, College of Health Sciences, University of Ghana, Accra, Ghana.
Beverly EgyirBacteriology Department, Noguchi Memorial Institute for Medical Research, University of Ghana, Accra, Ghana.
Benjamin D NuerteyCommunity Health Department, University of Ghana Medical School, College of Health Sciences, University of Ghana, Accra, Ghana.
George HedidorWorld Health Organization- Ghana Office, Ghana.ORCID 0000-0002-3147-5143
Gifty BoatengNational Public Health and Reference Laboratory, Korle Bu, Ghana.
Kwaku Asah-OpokuDepartment of Obstetrics and Gynaecology, University of Ghana Medical School, College of Health Sciences, University of Ghana, Accra, Ghana.
Thomas DankwahDepartment of Microbiology, Korle Bu Teaching Hospital, Korle Bu, Ghana.
Esther OkineDepartment of Microbiology, Korle Bu Teaching Hospital, Korle Bu, Ghana.
Japheth A OpintanDepartment of Medical Microbiology, University of Ghana Medical School, College of Health Sciences, University of Ghana, Accra, Ghana.ORCID 0000-0003-3928-2116

Funding

World Health Organization 001
6 · The paper itself

Abstract

backgroundVulnerable groups, such as pregnant women, are at increased risk of potentially life-threatening infections with extended-spectrum beta-lactamase-producing Enterobacterales (ESBL-E) for both mother and newborn. However, data regarding ESBL-E carriage and associated risk factors in Ghanaian pregnant women remain scarce.

objectiveThis study aimed to determine the prevalence of ESBL-E carriage and its associated risk factors among pregnant women attending the antenatal clinic at the Korle Bu Teaching Hospital.

methodsA systematic sample of 700 pregnant women with gestational age ≥ 34 weeks attending the antenatal clinic at Korle Bu Teaching Hospital was included in the study. After administering a structured questionnaire to assess potential risk factors associated with ESBL-E carriage, patients were given a sterile stool container to submit at least 1 g of stool specimen. Recovered isolates from faecal specimens were identified using MALDI-TOF-MS technology. These isolates were then subjected to susceptibility testing and ESBL identification. A random subset of 24 ESBL-producing Escherichia coli isolates was whole-genome sequenced on the MiSeq Illumina platform. Risk factors associated with ESBL-E carriage were determined using multivariable logistic regression analysis.

resultsAmong the 700 pregnant women, 42% (294) carried ESBL-E. The predominant ESBL-producing Enterobacterales were Escherichia coli (95%). Fifty percent (50%) of ESBL-E were multidrug resistant isolates (MDRs). Whole-genome sequencing of 24 ESBL-producing E. coli isolates revealed that blaCTX-M-15 (96%) was the most prevalent ESBL gene type. Notably, most isolates belonged to commensal phylogenetic groups (A, B1, and C; 88%). Having a primary level of education (aOR 1.45, 95% CI 1.05-1.96) and consuming legumes as the main source of protein (aOR 0.17, 0.40-0.83) were significantly associated with intestinal carriage of ESBL-E.

conclusionThis study identified a high prevalence of ESBL-E and MDR-ESBL-E carriage among pregnant women. Our findings underscore the urgent need for public health interventions to control the spread of AMR.

Indexed as

beta-LactamasesAdolescentAdultAnti-Bacterial AgentsEnterobacteriaceaeEnterobacteriaceae InfectionsEscherichia coliFecesFemaleGhanaHumansMicrobial Sensitivity TestsPregnancyPrevalenceRisk FactorsYoung AdultAnti-Bacterial Agentsbeta-Lactamases

Identifiers

PMID39527553
PMCPMC11554194

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LicenceCC BY
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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.