Evidence map›Paper›PMID 39527358›Full record

ArticleClinical & translational oncology : official publication of the Federation of Spanish Oncology Societies and of the National Cancer Institute of Mexico2025

XRCC1 is linked to poor prognosis in adenocarcinoma of the esophagogastric junction after radiotherapy: transcriptome and alternative splicing events analysis.

Pengfei Lu, Min Xia, Juan Li, Hongzhi Qi, Hui Wang, Rui Mao

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Article in Clinical & translational oncology : official publication of the Federation of Spanish Oncology Societies and of the National Cancer Institute of Mexico, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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1 · What the graph read from it

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3 · Its place in the literature

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4 · The record

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5 · Who and what money

Authors and funding

6 authors.

Pengfei LuCancer Center, The First Affiliated Hospital of Xinjiang Medical University, No. 137 Liyushan Road, Urumqi, 830054, Xinjiang, China.
Min XiaCancer Center, The First Affiliated Hospital of Xinjiang Medical University, No. 137 Liyushan Road, Urumqi, 830054, Xinjiang, China.
Juan LiDepartment of Infectious Diseases, The First People's Hospital of Urumqi, Urumqi, 830000, China.
Hongzhi QiCancer Center, The First Affiliated Hospital of Xinjiang Medical University, No. 137 Liyushan Road, Urumqi, 830054, Xinjiang, China.
Hui WangState Key Laboratory of Pathogenesis, Prevention and Treatment of High Incidence Diseases in Central Asia, Clinical Medicine Institute, The First Affiliated Hospital of Xinjiang Medical University, Urumqi, 830054, Xinjiang, China. wangh0923@126.com.
Rui MaoCancer Center, The First Affiliated Hospital of Xinjiang Medical University, No. 137 Liyushan Road, Urumqi, 830054, Xinjiang, China. maorui44@sohu.com.ORCID http://orcid.org/0000-0003-2846-8003

Funding

The Natural Science Foundation of Xinjiang Uygur Autonomous Region Outstanding Youth Project 2022D01E074Xinjiang Uygur Autonomous Region Tianshan Youth Plan Outstanding young scientific and technological talents training project 2020Q008
6 · The paper itself

Abstract

purposeThis study aimed to (i) investigate the relationship between X-ray repair cross-complementing protein 1 gene (XRCC1) and prognosis in patients with adenocarcinoma of the esophagogastric junction (AEG), and (ii) analyze the roles of XRCC1 in human gastric adenocarcinoma (AGS) cells following X-ray radiation.

methodsA total of 46 AEG patients were enrolled and examined for XRCC1 protein by immunohistochemistry. XRCC1 was knocked down in AGS cells by transfection, and AGS cells were subsequently exposed to 6 Gy of X-ray radiation. XRCC1 mRNA and protein expression was examined via quantitative real-time PCR (qRT-PCR) and Western blot analysis. The apoptosis of AGS cells was examined by flow cytometer. RNA-sequencing technology was used to identified differentially expressed genes and alternative splicing events following XRCC1 knockdown and radiation exposure.

resultsXRCC1 positivity was strongly associated with distant metastasis, pathological tumor-node-metastasis (pTNM) classification, and radiotherapy resistance in AEG patients. A significant difference in progression-free survival was observed between AEG patients with low and high XRCC1 protein expression. The knockdown of XRCC1 notably exacerbated the effects of X-ray radiation on apoptosis in AGS cells. Additionally, X-ray radiation modified the expression of genes related to apoptosis and immune response in XRCC1-knockdown AGS cells. Furthermore, the generation of splice variants was influenced by XRCC1 knockdown in AGS cells.

conclusionXRCC1 may serve as a key oncogene that elucidates the role of alternative splicing events in the progression of AEG following X-ray treatment.

Indexed as

AdenocarcinomaAlternative SplicingEsophageal NeoplasmsEsophagogastric JunctionStomach NeoplasmsTranscriptomeX-ray Repair Cross Complementing Protein 1AgedApoptosisCell Line, TumorFemaleHumansMaleMiddle AgedPrognosisRadiation ToleranceX-ray Repair Cross Complementing Protein 1XRCC1 protein, humanAdenocarcinoma of the esophagogastric junctionAlternative splicingPrognosisRadiotherapyXRCC1

Identifiers

PMID39527358

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.