Evidence map›Paper›PMID 39527222›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2025

Imaging Mass Cytometry to Decipher the Maturation of Tertiary Lymphoid Structures.

Marion Le Rochais, Sarah Ghamry-Barrin, Marylou Panouillot, Patrice Hémon, Soizic Garaud

Abstract read
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In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Review
  2. Review
  3. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Marion Le RochaisLBAI, UMR1227, University of Brest, Inserm, Brest, France.
Sarah Ghamry-BarrinInserm U1135, Paris, France.
Marylou PanouillotInserm U1135, Paris, France.
Patrice HémonLBAI, UMR1227, University of Brest, Inserm, Brest, France.
Soizic GaraudLBAI, UMR1227, University of Brest, Inserm, Brest, France. soizic.garaud@univ-brest.fr.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

In the realm of inflammation, including conditions like cancers, infections, and autoimmune diseases, the emergence of tertiary lymphoid structures (TLS) holds significant implications for prognosis and treatment response. Yet, traditional methodologies such as immunohistochemistry and immunofluorescence falter in capturing the nuanced complexities of TLS, necessitating innovative approaches, like imaging mass cytometry (IMC), to unravel their significance. With the capacity to concurrently assess nearly 40 markers within the same tissue section, IMC transcends the constraints of traditional approaches.This chapter delineates the IMC methodology tailored for TLS visualization and subsequent quantification. By leveraging IMC on formalin-fixed, paraffin-embedded (FFPE) sections of human colorectal cancer (CRC) tissues, we aim to establish a standardized protocol that elucidates the intricacies of TLS dynamics and composition.

Indexed as

Colorectal NeoplasmsImage CytometryTertiary Lymphoid StructuresHumansParaffin EmbeddingAlcian blueHESHistological stainingImage analysisImaging mass cytometryImmunohistochemistryTertiary lymphoid structure

Identifiers

PMID39527222

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.