Evidence map›Paper›PMID 39527209›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2025

Label-Free Quantitative Phosphoproteomics in the Fission Yeast Schizosaccharomyces pombe.

Barbara Sivakova, Jan Jurcik, Veronika Lukacova, Laura Olivia Lalakova, Tomas Selicky, Ingrid Cipakova, Peter Barath, Lubos Cipak

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Article in Methods in molecular biology (Clifton, N.J.), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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0cells of the map it votes in
0citing papers in PubMed
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1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Barbara SivakovaInstitute of Chemistry, Slovak Academy of Sciences, Bratislava, Slovakia.
Jan JurcikCancer Research Institute, Biomedical Research Center, Slovak Academy of Sciences, Bratislava, Slovakia.
Veronika LukacovaMedirex Group Academy n.o, Nitra, Slovakia.
Laura Olivia LalakovaCancer Research Institute, Biomedical Research Center, Slovak Academy of Sciences, Bratislava, Slovakia.
Tomas SelickyCancer Research Institute, Biomedical Research Center, Slovak Academy of Sciences, Bratislava, Slovakia.
Ingrid CipakovaCancer Research Institute, Biomedical Research Center, Slovak Academy of Sciences, Bratislava, Slovakia.
Peter BarathInstitute of Chemistry, Slovak Academy of Sciences, Bratislava, Slovakia.
Lubos CipakCancer Research Institute, Biomedical Research Center, Slovak Academy of Sciences, Bratislava, Slovakia. lubos.cipak@savba.sk.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Protein phosphorylation is a dynamic, reversible posttranslational modification that plays an important role in the regulation of cell signaling. Recently, label-free quantitative (LFQ) phosphoproteomics has become a powerful tool to analyze the phosphorylation of proteins within complex samples. In this chapter, we describe how to apply LFQ phosphoproteomics that is based on Fe-IMAC phosphopeptide enrichment followed by strong anion exchange (SAX) and porous graphitic carbon (PGC) fractionation strategies for identification and quantification of changes in the phosphoproteome in the fission yeast Schizosaccharomyces pombe.

Indexed as

PhosphoproteinsProteomicsSchizosaccharomycesSchizosaccharomyces pombe ProteinsPhosphopeptidesPhosphorylationProtein Processing, Post-TranslationalProteomePhosphopeptidesPhosphoproteinsProteomeSchizosaccharomyces pombe ProteinsLFQ phosphoproteomicsMass spectrometryPGC fractionationPhosphopeptide enrichmentSAX fractionationSchizosaccharomyces pombe

Identifiers

PMID39527209

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.