Evidence map›Paper›PMID 39527207›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2025

Dissecting the Cell-Killing Mechanisms of Hydroxyurea Using Spot Assays.

Kamal Dev, Ilknur Yurtsever, Sankhadip Bhadra, Yeseswi A Guduri, Kajal Davi, Yong-Jie Xu

Abstract read
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In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Kamal DevDepartment of Pharmacology and Toxicology, Boonshoft School of Medicine, Wright State University, Dayton, OH, USA.
Ilknur YurtseverDepartment of Pharmacology and Toxicology, Boonshoft School of Medicine, Wright State University, Dayton, OH, USA.
Sankhadip BhadraDepartment of Pharmacology and Toxicology, Boonshoft School of Medicine, Wright State University, Dayton, OH, USA.
Yeseswi A GuduriDepartment of Pharmacology and Toxicology, Boonshoft School of Medicine, Wright State University, Dayton, OH, USA.
Kajal DaviDepartment of Pharmacology and Toxicology, Boonshoft School of Medicine, Wright State University, Dayton, OH, USA.
Yong-Jie XuDepartment of Pharmacology and Toxicology, Boonshoft School of Medicine, Wright State University, Dayton, OH, USA. yong-jie.xu@wright.edu.

Funding

DNA Replication Checkpoint in Fission YeastR35GM144307 · NIGMS · WRIGHT STATE UNIVERSITY · PI Yongjie Xu · 2022 to 2026
$1.9M
NIGMS NIH HHS R35 GM144307
6 · The paper itself

Abstract

Hydroxyurea is an inhibitor of ribonucleotide reductase and is commonly used in laboratories to induce replication stress or arrest cells in the S phase for cell cycle or checkpoint studies. However, hydroxyurea also causes side effects such as oxidative stress, particularly under chronic exposure conditions. This complicates the interpretation of the cell-killing mechanisms, particularly in a previously uncharacterized mutant, and thus hampers the analyses. Here, we describe a few easy and simple spot assays in fission yeast that allow dissecting the cell-killing mechanisms of hydroxyurea.

Indexed as

HydroxyureaSchizosaccharomycesOxidative StressHydroxyureaAnaerobic cell cultureCell cycleDNA damage checkpointDNA replication checkpointGuanazoleHydroxyureaOxidative stressReplication stressRibonucleotide reductase

Identifiers

PMID39527207
PMCPMC11667678

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.