Evidence map›Paper›PMID 39527203›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2025

A Rapidly Inducible DNA Double-Strand Break to Monitor Telomere Formation, DNA Repair, and Checkpoint Activation.

Haitao Zhang, Carly Kerr, Julien Audry, Kurt W Runge

Abstract read
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In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Haitao ZhangDepartment of Inflammation and Immunity, Lerner Research Institute, Cleveland Clinic Foundation, Cleveland, OH, USA.
Carly KerrDepartment of Inflammation and Immunity, Lerner Research Institute, Cleveland Clinic Foundation, Cleveland, OH, USA.
Julien AudryDepartment of Inflammation and Immunity, Lerner Research Institute, Cleveland Clinic Foundation, Cleveland, OH, USA.
Kurt W RungeDepartment of Inflammation and Immunity, Lerner Research Institute, Cleveland Clinic Foundation, Cleveland, OH, USA. rungek@ccf.org.

Funding

Mechanisms controlling the efficiency of hemostatic vitamin K-dependent protein activationR01HL158007 · NHLBI · CLEVELAND CLINIC LERNER COM-CWRU · PI BERKNER, KATHLEEN LUCILE, RUNGE, KURT W · 2021 to 2024
$2.2M
Impact of gamma-glutamyl carboxylase processivity on vitamin K-dependent protein modification and function in human health and diseaseR01HL152678 · NHLBI · CLEVELAND CLINIC LERNER COM-CWRU · PI BERKNER, KATHLEEN LUCILE, RUNGE, KURT W · 2021 to 2024
$2.1M
NHLBI NIH HHS R01 HL152678NHLBI NIH HHS R01 HL158007
6 · The paper itself

Abstract

The study of processes that govern genome integrity has been augmented by the ability to create a precise DNA double-strand break (DSB) in a short period of time that allows the kinetics of DNA metabolism and protein recruitment to be followed. Defined DSBs are made by expressing endonucleases with long recognition sites that are rare or absent in the genome, and require that the endonuclease is only active when induced. Research in this area in Schizosaccharomyces pombe was limited because rapidly inducible promoters were not available until around 2005, and several rapidly inducible DSB systems are now available. Here, we describe a system to rapidly induce a modified I-SceI endonuclease that can generate a DSB 20 min after induction. I-SceI has no recognition sites in the S. pombe genome, allowing the introduction of complex substrates to monitor the effects of a new DSB in real time. This chapter describes how I-SceI can be most efficiently induced and a simple cell length measurement assay to monitor cell cycle checkpoint activation from a single DSB.

Indexed as

DNA Breaks, Double-StrandedDNA RepairSchizosaccharomycesTelomereCell Cycle CheckpointsDeoxyribonucleases, Type II Site-SpecificDNA, FungalSchizosaccharomyces pombe ProteinsDeoxyribonucleases, Type II Site-SpecificDNA, FungalSchizosaccharomyces pombe ProteinsCell lengthG2/M checkpointInducible telomere formationI-SceI nucleaseProto-telomereSchizosaccharomyces pombe (S. pombe)Tet-promoter

Identifiers

PMID39527203

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.