Evidence map›Paper›PMID 39527198›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2025

Fast Fission Yeast Genome Editing by CRISPR/Cas9 Using Gap Repair and Fluoride Selection.

Yuan Ren, Ronan Fernandez, Takumi Saito, Barbara Fujita, Iman Mousavi, Julien Berro

Abstract read
PubMed Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Yuan RenDepartment of Molecular Biophysics and Biochemistry, Yale University, New Haven, CT, USA.
Ronan FernandezDepartment of Molecular Biophysics and Biochemistry, Yale University, New Haven, CT, USA.
Takumi SaitoDepartment of Molecular Biophysics and Biochemistry, Yale University, New Haven, CT, USA.
Barbara FujitaDepartment of Molecular Biophysics and Biochemistry, Yale University, New Haven, CT, USA.
Iman MousaviDepartment of Molecular Biophysics and Biochemistry, Yale University, New Haven, CT, USA.
Julien BerroDepartment of Molecular Biophysics and Biochemistry, Yale University, New Haven, CT, USA. julien.berro@yale.edu.

Funding

Molecular mechanisms of force production and force sensing during clathrin-mediated endocytosisR01GM115636 · NIGMS · YALE UNIVERSITY · PI Julien Berro · 2016 to 2026
$4.7M
New strategy to measure forces at the molecular scale in vivoR21GM132661 · NIGMS · YALE UNIVERSITY · PI BERRO, JULIEN · 2019 to 2020
$461k
NIGMS NIH HHS R01 GM115636NIGMS NIH HHS R21 GM132661
6 · The paper itself

Abstract

We present a protocol to perform CRISPR/Cas9-mediated genome editing in the fission yeast Schizosaccharomyces pombe that does not require cloning and uses the fluoride exporter channel Fex1 as the selection marker. Transformation is typically carried out on the same day of PCR primer arrival and successfully edited strains are selected 5 days after transformation. We expect the adoption of this protocol to further accelerate the throughput of genome editing in S. pombe.

Indexed as

CRISPR-Cas SystemsGene EditingSchizosaccharomycesFluoridesGenome, FungalFluoridesCRISPRFission yeastFluorideGenome editing

Identifiers

PMID39527198

What OpenQuestion holds

Textmetadata
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.