Evidence map›Paper›PMID 39527196›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2025

Bimolecular Fluorescence Complementation as a Tool to Study Specific Dynamic Interactions Between Proteins in Fission Yeast.

Emilio Gonzalez-Martin, Victor A Tallada

Abstract read
PubMed Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

2 authors.

Emilio Gonzalez-MartinCentro Andaluz de Biología del Desarrollo, Universidad Pablo de Olavide-Consejo Superior de Investigaciones Científicas-Junta de Andalucía, Seville, Spain.
Victor A TalladaCentro Andaluz de Biología del Desarrollo, Universidad Pablo de Olavide-Consejo Superior de Investigaciones Científicas-Junta de Andalucía, Seville, Spain. valvtal@upo.es.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Bimolecular fluorescence complementation (BiFC) is a technique that enables real-time observation within living cells of the interaction between two proteins forming a complex, determining the location where such interaction occurs within the cell, and even the association and dissociation cycles in response to physiological cues. Here, we describe in detail the use of bimolecular fluorescence complementation to visualize the assembly and disassembly of cohesin over the fission yeast cell cycle.

Indexed as

Cell Cycle ProteinsSchizosaccharomycesCell CycleChromosomal Proteins, Non-HistoneCohesinsMicroscopy, FluorescenceProtein BindingProtein Interaction MappingSchizosaccharomyces pombe ProteinsCell Cycle ProteinsChromosomal Proteins, Non-HistoneCohesinsSchizosaccharomyces pombe ProteinsBimolecular fluorescence complementationCell cycleCohesinLive-cell imagingProtein–protein interaction

Identifiers

PMID39527196

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.