Global neural encoding of behavioral strategies in mice during perceptual decision-making task with two different sensory patterns.

When a simple model-free strategy does not provide sufficient outcomes, an inference-based strategy estimating a hidden task structure becomes essential for optimizing choices. However, the neural circuitry involved in inference-based strategies is still unclear. We developed a tone frequency discrimination task in head-fixed mice in which the tone category of the current trial depended on the category of the previous trial. When the tone category was repeated, the mice continued using the default model-free strategy, as well as when the tone was randomly presented, to bias choices. In contrast, when the tone was alternated, the default strategy gradually shifted to a hybrid of model-free and inference-based strategies, although we did not observe distinct strategy changes. Brain-wide electrophysiological recording suggested that the neural activity of the frontal and sensory cortices, hippocampus, and striatum was correlated with the reward expectation in different task conditions, suggesting the global encoding of multiple strategies in the brain.