Evidence map›Paper›PMID 39523734›Full record

ReviewSmall (Weinheim an der Bergstrasse, Germany)2025

Exploring Biomineralization Processes Using In Situ Liquid Transmission Electron Microscopy: A Review.

Liza-Anastasia DiCecco, Tengteng Tang, Eli D Sone, Kathryn Grandfield

Abstract readReview
In one paragraph

Review in Small (Weinheim an der Bergstrasse, Germany), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed.

  1. Article
  2. Article
  3. Article
  4. Article
  5. Review
  6. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Liza-Anastasia DiCeccoDepartment of Materials Science and Engineering, McMaster University, Hamilton, ON, L8S 4L7, Canada.ORCID 0000-0001-8420-1114
Tengteng TangDepartment of Materials Science and Engineering, McMaster University, Hamilton, ON, L8S 4L7, Canada.ORCID 0000-0002-6417-5892
Eli D SoneInstitute of Biomedical Engineering, University of Toronto, Toronto, ON, M5S 3G9, Canada.ORCID 0000-0001-7087-473X
Kathryn GrandfieldDepartment of Materials Science and Engineering, McMaster University, Hamilton, ON, L8S 4L7, Canada.ORCID 0000-0002-0188-9580

Funding

Canadian Institutes of Health Research Project Grant Program 180575Natural Sciences and Engineering Research Council of Canada (NSERC) Discovery Grant program RGPIN-2020-05722
6 · The paper itself

Abstract

Liquid transmission electron microscopy (TEM) is a newly established technique broadly used to study reactions in situ. Since its emergence, complex and multifaceted biomineralization processes have been revealed with real-time resolution, where classical and non-classical mineralization pathways have been dynamically observed primarily for Ca and Fe-based mineral systems in situ. For years, classical crystallization pathways have dominated theories on biomineralization progression despite observations of non-traditional routes involving precursor phases using traditional- and cryo-TEM. The new dynamic lens provided by liquid TEM is a key correlate to techniques limited to time-stamped, static observations - helping shift paradigms in biomineralization toward non-classical theories with dynamic mechanistic visualization. Liquid TEM provides new insights into fundamental biomineralization processes and essential physiological and pathological processes for a wide range of organisms. This review critically reviews a summary of recent in situ liquid TEM research related to the biomineralization field. Key liquid TEM preparation and imaging parameters are provided as a foundation for researchers while technical challenges are discussed. In future, the expansion of liquid TEM research in the biomineralization field will lead to transformative discoveries, providing complementary dynamic insights into biological systems.

Indexed as

BiomineralizationMicroscopy, Electron, TransmissionMineralsMineralsamorphous precursorsbiomineralization, biominerals, crystallizationliquid electron microscopy

Identifiers

PMID39523734
PMCPMC11735904

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.