Evidence map›Paper›PMID 39523301›Full record

ArticleJournal of nanobiotechnology2024

Ion osmolarity-driven sequential concentration-enrichment for the scale-up isolation of extracellular vesicles.

Lizhi Wang, Junhao Xia, Xin Guan, Yang Song, Mengru Zhu, Fengya Wang, Baofeng Zhao, Lukuan Liu, Jing Liu

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of nanobiotechnology, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Lizhi WangStem Cell Clinical Research Center, The First Affiliated Hospital of Dalian Medical University, Dalian, 116011, China.
Junhao XiaStem Cell Clinical Research Center, The First Affiliated Hospital of Dalian Medical University, Dalian, 116011, China.
Xin GuanStem Cell Clinical Research Center, The First Affiliated Hospital of Dalian Medical University, Dalian, 116011, China.
Yang SongStem Cell Clinical Research Center, The First Affiliated Hospital of Dalian Medical University, Dalian, 116011, China.
Mengru ZhuStem Cell Clinical Research Center, The First Affiliated Hospital of Dalian Medical University, Dalian, 116011, China.
Fengya WangStem Cell Clinical Research Center, The First Affiliated Hospital of Dalian Medical University, Dalian, 116011, China.
Baofeng ZhaoState Key Laboratory of Medical Proteomics, National Chromatographic R. & A. Center, CAS Key Laboratory of Separation Science for Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, 116023, China.
Lukuan LiuStem Cell Clinical Research Center, The First Affiliated Hospital of Dalian Medical University, Dalian, 116011, China. lukuanliu@163.com.
Jing LiuStem Cell Clinical Research Center, The First Affiliated Hospital of Dalian Medical University, Dalian, 116011, China. liujing@dmu.edu.cn.

Funding

DMU-1&DICP DMU-1&DICP UN202204the Dalian High-level Talent Team Project 2022RG18the Liaoning Directed Project for Planning of Science and Technology 2021JH2/10300135the National Natural Science Foundation of China 22004012
6 · The paper itself

Abstract

Extracellular vesicles (EVs) carry a variety of bioactive molecules and are becoming a promising alternative to cell therapy. Scale-up EV isolation is necessary for their functional studies and biological applications, while the traditional methods are challenged by low throughput, low yield, and potential damage. Herein, we developed an ion osmolarity-driven sequential concentration-enrichment strategy (IOSCE) for the EV isolation. IOSCE is composed of a novel superabsorbent polymers (SAPs) for EV concentration and a charged polymer for EV enrichment. Based on the driving force of ionic osmotic pressure, IOSCE can isolate EVs on a large scale from cell culture medium. The saturated water absorption capacity of IOSCE is 13.62 times higher than that of commercial SAPs. Compared with the ultracentrifugation method, IOSCE exhibited a 2.64 times higher yield (6.33 × 10

Indexed as

Extracellular VesiclesMesenchymal Stem CellsAnimalsHumansIonsOsmolar ConcentrationOsmotic PressurePolymersIonsPolymersExtracellular vesiclesIsolationNeuroinflammationProteomeStem cell

Identifiers

PMID39523301
PMCPMC11550536

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.