Evidence map›Paper›PMID 39523223›Full record

ArticleOdontology2025

Metronidazole-laden silk fibroin methacrylated scaffolds for managing periapical lesions.

Ashley Silverberg, Lais M Cardoso, Ana Beatriz G de Carvalho, Alexandre H Dos Reis-Prado, J Christopher Fenno, Renan Dal-Fabbro, Marco C Bottino

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Odontology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Bilayer gelatin-methacryloyl scaffold for pulp inflammation suppression and dentin-like tissue regeneration.Journal of controlled release : official journal of the Controlled Release Society · 2026
    Article
  2. Article
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Ashley SilverbergDepartment of Cariology, Restorative Sciences and Endodontics, University of Michigan, School of Dentistry, 1011 N. University Avenue, Room 2303, Ann Arbor, MI, 48109, USA.
Lais M CardosoDepartment of Cariology, Restorative Sciences and Endodontics, University of Michigan, School of Dentistry, 1011 N. University Avenue, Room 2303, Ann Arbor, MI, 48109, USA.
Ana Beatriz G de CarvalhoDepartment of Cariology, Restorative Sciences and Endodontics, University of Michigan, School of Dentistry, 1011 N. University Avenue, Room 2303, Ann Arbor, MI, 48109, USA.
Alexandre H Dos Reis-PradoDepartment of Cariology, Restorative Sciences and Endodontics, University of Michigan, School of Dentistry, 1011 N. University Avenue, Room 2303, Ann Arbor, MI, 48109, USA.
J Christopher FennoDepartment of Biologic and Materials Sciences & Prosthodontics, University of Michigan, School of Dentistry, 1011 N. University Avenue, Ann Arbor, MI, 48109, USA.
Renan Dal-FabbroDepartment of Cariology, Restorative Sciences and Endodontics, University of Michigan, School of Dentistry, 1011 N. University Avenue, Room 2303, Ann Arbor, MI, 48109, USA.
Marco C BottinoDepartment of Cariology, Restorative Sciences and Endodontics, University of Michigan, School of Dentistry, 1011 N. University Avenue, Room 2303, Ann Arbor, MI, 48109, USA. mbottino@umich.edu.ORCID http://orcid.org/0000-0001-8740-2464

Funding

Personalized Strategies for Periodontal Tissue Regeneration - A Converged Biofabrication ApproachR01DE031476 · NIDCR · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI Marco C Bottino · 2022 to 2026
$2.6M
NIDCR NIH HHS R01 DE031476NIDCR NIH HHS R01DE031476
6 · The paper itself

Abstract

This study aimed to develop and characterize silk fibroin methacrylated/SilkMA electrospun scaffolds associated with metronidazole/MET to control infection in root-end resected periapical lesions while supporting bone regeneration. SilkMA-based formulations (10% w/v) incorporating MET (0-control; 5, 15, or 30% w/w) were electrospun into fibrous scaffolds and photocrosslinked. Scaffolds' morphology, chemical composition, swelling/degradation profiles, mechanical properties, cytocompatibility with alveolar bone-derived mesenchymal stem cells/aBMSCs and stem cells from apical papilla/SCAPs, anti-inflammatory potential, and antibacterial efficacy (direct contact assay against Aggregatibacter actinomycetemcomitans/Aa and Fusobacterium nucleatum/Fn; Aa biofilm model) were assessed. Statistical analysis was conducted using a significance level of 5%. Morphological analysis revealed that MET content influenced fiber diameters post-crosslinking, while the chemical composition analysis confirmed MET integration within the scaffolds. 30%MET-laden scaffolds demonstrated reduced swelling capacity compared to SilkMA/control scaffolds, while complete degradation was observed after 42 days for the formulated scaffolds. Mechanical testing indicated enhanced stiffness and tensile strength in 30%MET-laden scaffolds compared to SilkMA/control (p < 0.05). Cytocompatibility evaluations showed non-cytotoxic effects across all formulations for aBMSCs and SCAPs. Anti-inflammatory assays demonstrated decreased pro-inflammatory cytokine interleukin-6 synthesis by aBMSCs treated with SilkMA + MET30% and Escherichia coli LPS, comparable to negative control (p > 0.05). Antibacterial efficacy assays revealed significant inhibition of Aa and Fn, with 30%MET-laden scaffolds demonstrating biofilm inhibition against Aa (p < 0.05). These findings underscore the potential of SilkMA scaffolds laden with MET as a promising strategy for managing periapical lesions, offering enhanced structural support, antimicrobial properties, and biocompatibility crucial for effective tissue regeneration and infection control after endodontic surgery.

Indexed as

Anti-Infective AgentsFibroinsMethacrylatesMetronidazolePeriapical DiseasesTissue ScaffoldsAggregatibacter actinomycetemcomitansAnimalsAnti-Bacterial AgentsBiofilmsFusobacterium nucleatumHumansMesenchymal Stem CellsAnti-Bacterial AgentsAnti-Infective AgentsFibroinsMethacrylatesMetronidazoleApical periodontitisApicoectomyMetronidazoleSilkTissue scaffolds

Identifiers

PMID39523223
PMCPMC13064872

What OpenQuestion holds

Textmetadata
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Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.