Evidence map›Paper›PMID 39523168›Full record

ArticleUltrasound in medicine & biology2025

Ischemia/Reperfusion Injury Enhances Accumulation of Perfluoropropane Droplets.

Cheng Chen, Shouqiang Li, Terry O Matsunaga, John J Pacella, E Carr Everbach, Feng Xie, Thomas R Porter, Flordeliza S Villanueva, Xucai Chen

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In one paragraph

Article in Ultrasound in medicine & biology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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1 · What the graph read from it

What it found

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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

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3 · Its place in the literature

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4 · The record

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5 · Who and what money

Authors and funding

9 authors.

Cheng ChenCenter for Ultrasound Molecular Imaging and Therapeutics, University of Pittsburgh, Pittsburgh, PA, USA.
Shouqiang LiDivision of Cardiovascular Medicine, University of Nebraska Medical Center, Omaha, NE, USA.
Terry O MatsunagaDepartment of Biomedical Engineering and Department of Medical Imaging, University of Arizona, Tucson, AZ, USA.
John J PacellaCenter for Ultrasound Molecular Imaging and Therapeutics, University of Pittsburgh, Pittsburgh, PA, USA.
E Carr EverbachSwarthmore College, Swarthmore, PA, USA.
Feng XieDivision of Cardiovascular Medicine, University of Nebraska Medical Center, Omaha, NE, USA.
Thomas R PorterDivision of Cardiovascular Medicine, University of Nebraska Medical Center, Omaha, NE, USA.
Flordeliza S VillanuevaCenter for Ultrasound Molecular Imaging and Therapeutics, University of Pittsburgh, Pittsburgh, PA, USA.
Xucai ChenCenter for Ultrasound Molecular Imaging and Therapeutics, University of Pittsburgh, Pittsburgh, PA, USA. Electronic address: chenxx2@upmc.edu.

Funding

Scar Detection and Treatment with Droplet ActivationR01HL146489 · NHLBI · UNIVERSITY OF NEBRASKA MEDICAL CENTER · PI PORTER, THOMAS R · 2020 to 2023
$3.0M
NHLBI NIH HHS R01 HL146489
6 · The paper itself

Abstract

objectivePerfluoropropane droplets (PD) are nanometer-sized particles that can be formulated from commercially available contrast agents. The preferential retention of PDs in diseased microvascular beds can be detected by ultrasound imaging techniques after acoustic activation and offers an opportunity for the detection of such processes as scar formation or inflammation. We hypothesized that in the presence of ischemia/reperfusion (I/R) injury, retention of intravenously injected PDs would be enhanced.

methodsUsing an established intravital microscopy model of rat cremaster microcirculation, we determined the retention and subsequent acoustic activation behavior of PDs in exteriorized rat cremaster tissue. DiI-labeled droplets (200 µL) were administered intravenously. Acoustic activation was achieved with a clinical ultrasound system at two ultrasound frequencies (1.5 and 7 MHz).

resultsFluorescent microbubbles could be detected in the microvasculature after intravenous injection of PDs and subsequent acoustic activation. Increased retention of PDs was observed in the I/R group compared with control group with both ultrasound frequencies (p < 0.05). Using higher-resolution microscopy, we found evidence that some droplets extravasate to the outside of the endothelial border or are potentially engulfed by leukocytes.

conclusionOur data indicate that targeted imaging of the developing scar zones might be possible with ultrasound activation of intravenously injected PDs, and a method of targeting therapies to these same regions could be developed.

Indexed as

FluorocarbonsReperfusion InjuryAnimalsContrast MediaDisease Models, AnimalMaleMicrobubblesRatsRats, Sprague-DawleyUltrasonographyContrast MediaFluorocarbonsperflutrenIschemia/reperfusionNon-invasive ultrasound imagingPerfluoropropane dropletsUltrasound contrast agents

Identifiers

PMID39523168
PMCPMC11744877

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.