Evidence map›Paper›PMID 39523095›Full record

ArticleNan fang yi ke da xue xue bao = Journal of Southern Medical University2024

[Cisplatin promotes TNF-α autocrine to trigger RIP1/RIP3/MLKL-dependent necroptosis of human head and neck squamous cell carcinoma cells].

H Wang, D Tao, J Ma, D Zhang, Z Shen, C Deng, J Zhou

Abstract readEnglish Abstract
PubMed Full text at PMC Publisher
In one paragraph

Article in Nan fang yi ke da xue xue bao = Journal of Southern Medical University, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
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1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

H WangSchool of Stomatology, Wannan Medical College, Wuhu 241002, China.
D TaoDepartment of Oral and Maxillofacial Surgery, First Affiliated Hospital (Yijishan Hospital) of Wannan Medical College, Wuhu 241100, China.
J MaSchool of Stomatology, Wannan Medical College, Wuhu 241002, China.
D ZhangSchool of Stomatology, Wannan Medical College, Wuhu 241002, China.
Z ShenSchool of Stomatology, Wannan Medical College, Wuhu 241002, China.
C DengSchool of Stomatology, Wannan Medical College, Wuhu 241002, China.
J ZhouSchool of Stomatology, Wannan Medical College, Wuhu 241002, China.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

objectiveTo investigate whether cisplatin induces tumor necrosis factor-α (TNF-α) secretion in human head and neck squamous cell carcinoma (HNSCC) cells to trigger RIP1/RIP3/MLKL-dependent necroptosis of the cells.

methodsHNSCC cell lines HN4 and SCC4 treated with cisplatin (CDDP) or the combined treatment with CDDP and z-VAD-fmk (a caspase inhibitor) or Nec-1 (a necroptosis inhibitor) for 24 h were examined for changes in cell viability using CCK8 assay and expressions of caspase-8 and necroptosis pathway proteins (RIP1/RIP3/MLKL) using Western blotting. The changes in migration of the cells were assessed with cell scratch assay, and the expressions of epithelial-mesenchymal transition (EMT) marker proteins N-cadherin, vimentin, and E-cadherin as well as the expressions of NF-κB (p65) and TNF-α were detected with Western blotting.

resultsThe IC

conclusionCisplatin activates nuclear factor-κB signaling in HNSCCs to promote TNF-α autocrine and induce RIP1/RIP3/MLKL-dependent necroptosis, thus leading to inhibition of cell proliferation.

Indexed as

CisplatinHead and Neck NeoplasmsNecroptosisProtein KinasesReceptor-Interacting Protein Serine-Threonine KinasesSquamous Cell Carcinoma of Head and NeckTumor Necrosis Factor-alphaApoptosisCarcinoma, Squamous CellCaspase 8Cell Line, TumorCell MovementCell SurvivalEpithelial-Mesenchymal TransitionHumansNuclear Pore Complex ProteinsAGFG1 protein, humanCaspase 8CisplatinMLKL protein, humanNuclear Pore Complex ProteinsProtein KinasesReceptor-Interacting Protein Serine-Threonine KinasesRIPK3 protein, humanRNA-Binding ProteinsTumor Necrosis Factor-alphacisplatinhuman head and neck squamous cell carcinomaNec-1necroptosisz-VAD-fmk

Identifiers

PMID39523095
PMCPMC11526464

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.