Evidence map›Paper›PMID 39520225›Full record

ArticleDevelopment, growth & differentiation2024

Labeling and sorting of avian primordial germ cells utilizing Lycopersicon Esculentum lectin.

Hiroko Iikawa, Aika Nishina, Mizuki Morita, Yuji Atsuta, Yoshiki Hayashi, Daisuke Saito

Abstract read
In one paragraph

Article in Development, growth & differentiation, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Hiroko IikawaGraduate School of Systems Life Sciences, Kyushu University, Fukuoka, Japan.
Aika NishinaGraduate School of Systems Life Sciences, Kyushu University, Fukuoka, Japan.
Mizuki MoritaGraduate School of Systems Life Sciences, Kyushu University, Fukuoka, Japan.
Yuji AtsutaGraduate School of Systems Life Sciences, Kyushu University, Fukuoka, Japan.ORCID https://orcid.org/0000-0002-5116-0800
Yoshiki HayashiGraduate School of Systems Life Sciences, Kyushu University, Fukuoka, Japan.
Daisuke SaitoGraduate School of Systems Life Sciences, Kyushu University, Fukuoka, Japan.ORCID https://orcid.org/0000-0002-5999-2505

Funding

Japan Society for the Promotion of Science 22H02634Japan Society for the Promotion of Science 24K21974
6 · The paper itself

Abstract

Avian species are essential resources for human society, with their preservation and utilization heavily dependent on primordial germ cells (PGCs). However, efficient methods for isolating live PGCs from embryos remain elusive in avian species beyond chickens, and even in chickens, existing techniques have shown limited efficiency. In this study, we present a rapid, simple, and cost-effective method for labeling and sorting circulating-stage PGCs across various avian species, including Carinatae and Ratitae, using Lycopersicon Esculentum (Tomato) lectin (LEL). Notably, this method demonstrates high sorting efficiency by identifying a wide range of PGC subtypes while preserving the proliferative and migratory potential of chicken PGCs. This approach is anticipated to significantly contribute to the conservation, research, and agricultural industries related to avian species globally.

Indexed as

Germ CellsAnimalsBirdsChickensPlant LectinsPlant LectinsAvianlycopersicon esculentum lectinprimordial germ cellsorting

Identifiers

PMID39520225
PMCPMC11659090

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.