Elucidation of the Effect of Solar Light on the Near-Infrared Excitation Raman Spectroscopy-Based Analysis of Fabric Dyes.

Colored textiles are valuable physical evidence often found at crime scenes. Analysis of the chemical structure of textiles could be used to establish a connection between fabric found at a crime scene and suspect cloths. High-performance liquid chromatography (HPLC) and mass spectroscopy coupled HPLC are traditionally used for the identification of dyes in fabric. However, these techniques are invasive and destructive. A growing body of evidence indicates that near-infrared excitation (λ = 830 nm) Raman spectroscopy (NIeRS) could be used to probe the chemical signature of such colorants. At the same time, it remains unclear whether environmental factors, such as solar light could lower the accuracy of NIeRS-based identification of dyes in textiles. In this study, we exposed cotton fabric colored with six different dyes to light and investigated the extent to which colorants fade during seven weeks using NIeRS. We found a decrease in the intensities of all vibrational bands in the acquired spectra as the time of the exposition of fabric to light increased. Nevertheless, utilization of partial least-squared discriminant analysis (PLS-DA) enabled identification of the colorants at all eight weeks. These results indicate that the effect of light exposure should be strongly considered by forensic experts upon the NIeRS-based analysis of colored fabric.