Evidence map›Paper›PMID 39518998›Full record

ArticleInternational journal of molecular sciences2024

Knockout of the Carbohydrate Responsive Element Binding Protein Enhances Proliferation and Tumorigenesis in Renal Tubules of Mice.

Kerrin Hansen, Kristin Peters, Christian K Burkert, Eric Brose, Diego F Calvisi, Katrina Ehricke, Maren Engeler, Elisa Knuth, Nils Kröger, Andrea Lohr and 7 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in International journal of molecular sciences, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

17 authors.

Kerrin HansenInstitut für Pathologie, Universitaetsmedizin Greifswald, DE-17489 Greifswald, Germany.
Kristin PetersInstitut für Pathologie, Universitaetsmedizin Greifswald, DE-17489 Greifswald, Germany.
Christian K BurkertInstitut für Pathologie, Universitaetsmedizin Greifswald, DE-17489 Greifswald, Germany.
Eric BroseInstitut für Pathologie, Universitaetsmedizin Greifswald, DE-17489 Greifswald, Germany.
Diego F CalvisiInstitut für Pathologie, Universität Regensburg, DE-93053 Regensburg, Germany.
Katrina EhrickeInstitut für Pathologie, Universitaetsmedizin Greifswald, DE-17489 Greifswald, Germany.
Maren EngelerInstitut für Pathologie, Universitaetsmedizin Greifswald, DE-17489 Greifswald, Germany.
Elisa KnuthInstitut für Pathologie, Universitaetsmedizin Greifswald, DE-17489 Greifswald, Germany.
Nils KrögerKlinik und Poliklinik für Urologie, Universitaetsmedizin Greifswald, DE-17489 Greifswald, Germany.
Andrea LohrInstitut für Pathologie, Universitaetsmedizin Greifswald, DE-17489 Greifswald, Germany.
Jessica PreyInstitut für Pathologie, Universitaetsmedizin Greifswald, DE-17489 Greifswald, Germany.
Jenny SonkeInstitut für Pathologie, Universitaetsmedizin Greifswald, DE-17489 Greifswald, Germany.
Padmanabhan VakeelInstitut für Pathologie, Universitaetsmedizin Greifswald, DE-17489 Greifswald, Germany.
Juliane WladaschInstitut für Pathologie, Universitaetsmedizin Greifswald, DE-17489 Greifswald, Germany.
Jenny ZimmerInstitut für Pathologie, Universitaetsmedizin Greifswald, DE-17489 Greifswald, Germany.
Frank DombrowskiInstitut für Pathologie, Universitaetsmedizin Greifswald, DE-17489 Greifswald, Germany.
Silvia RibbackInstitut für Pathologie, Universitaetsmedizin Greifswald, DE-17489 Greifswald, Germany.

Funding

Deutsche Forschungsgemeinschaft RI2695/1-3
6 · The paper itself

Abstract

Glycogen-storing so-called clear cell kidney tubules (CCTs), precursor lesions of renal cell carcinoma, have been described in diabetic rats and in humans. The lesions show upregulation of the Akt/mTOR-pathway and the related transcription factor carbohydrate responsive element binding protein (ChREBP), which is supposedly pro-oncogenic. We investigated the effect of ChREBP-knockout on nephrocarcinogenesis in streptozotocin-induced diabetic and normoglycemic mice. Diabetic, but not non-diabetic mice, showed CCTs at 3, 6 and 12 months of age. Glycogenosis was confirmed by periodic acid schiff reaction and transmission electron microscopy. CCTs in ChREBP-knockout mice consisted of larger cells and occurred more frequently compared to wildtype mice. Progression towards kidney tumors was observed in both diabetic groups but occurred earlier in ChREBP-knockout mice. Proliferative activity assessed by BrdU-labeling was lower in 1-week-old but higher in 12-month-old diabetic ChREBP-knockout mice. Surprisingly, renal neoplasms occurred spontaneously in non-diabetic ChREBP-knockout, but not non-diabetic wildtype mice, indicating an unexpected tumor-suppressive function of ChREBP. Immunohistochemistry showed upregulated glycolysis and lipogenesis, along with activated Akt/mTOR-signaling in tumors of ChREBP-knockout groups. Immunohistochemistry of human clear cell renal cell carcinomas revealed reduced ChREBP expression compared to normal kidney tissue. However, the molecular mechanisms by which loss of ChREBP might facilitate tumorigenesis require further investigation.

Indexed as

Basic Helix-Loop-Helix Leucine Zipper Transcription FactorsCarcinogenesisCell ProliferationDiabetes Mellitus, ExperimentalKidney NeoplasmsKidney TubulesMice, KnockoutAnimalsCarcinoma, Renal CellHumansMaleMiceBasic Helix-Loop-Helix Leucine Zipper Transcription FactorsMlxipl protein, mouseAkt/mTORclear cell tubulesglycogenMLXIPLnephrocarcinogenesisproliferationrenal cell carcinomastreptozotocintumor development

Identifiers

PMID39518998
PMCPMC11545909

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.