ArticleAnimals : an open access journal from MDPI2024
Impacts of Protein and Energy Levels on Rumen Fermentation and Microbial Activity Under Different Incubation Temperatures.
Article in Animals : an open access journal from MDPI, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
7 citing papers in PubMed.
- The influence of zinc oxide utilization in ruminants: A systematic review and meta-analysis.Veterinary and animal science · 2026Article
- Effects of Dietary Rumen-Undegradable Protein and Protein Levels on Growth Performance, Fermentation Parameters, Slaughter Performance, and Meat Quality on Fattening Hu Sheep.Animals : an open access journal from MDPI · 2026Article
- Nutritional Strategies to Mitigate Heat Stress in Cattle: A Narrative Review.Microorganisms · 2026Review
- Effects of Substitution of Corn withAnimals : an open access journal from MDPI · 2026Article
- Effects of L-Valine Supplementation in Low-Nitrogen Diets on Rumen Fermentation Parameters, Predicted Methane Emissions Production, and Microbial Communities In Vitro.Animals : an open access journal from MDPI · 2026Article
- Advances in Nutritional Manipulation of Rumen Fermentation.Animals : an open access journal from MDPI · 2025Article
- Microencapsulated Escape Lysine with Tannin as an Adjuvant in Sheep Diets.Veterinary sciences · 2025Article
Corrections and comments
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Authors and funding
6 authors.
Funding
Abstract
This study aimed to explore the effects of different incubation temperatures on ruminal fermentation and rumen microorganisms and determine the appropriate protein and energy levels to enhance microbial protein synthesis using an in vitro system. Rumen inoculum was collected from two fistulated Holstein heifers (trial 1: BW: 652.3 kg ± 25.2; trial 2: BW: 683.3 kg ± 30.2) and assessed using a closed-batch culture system. The experimental model employed a 2 × 5 factorial arrangement using incubation temperatures set to 39 and 41 °C, with protein levels set to 12.0, 13.5, 15.0, 16.5, and 18.0% of DM in trial 1 or with energy levels set to 2.4, 2.5, 2.6, 2.7, and 2.8 Mcal/kg of DM in trial 2. The data were analyzed using the MIXED procedure. The results showed increased (
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Registered trials
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