Evidence map›Paper›PMID 39517098›Full record

ArticleEnvironmental microbiology reports2024

Recovery of culturable Streptococcus pyogenes from swabs stored at different temperatures.

Kate Summer, Steven Y C Tong, Jonathan R Carapetis, Asha C Bowen

Abstract read
In one paragraph

Article in Environmental microbiology reports, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Kate SummerWesfarmers Centre for Vaccines and Infectious Diseases, Telethon Kids Institute, University of Western Australia, Nedlands, Western Australia, Australia.ORCID https://orcid.org/0000-0002-9013-1244
Steven Y C TongVictorian Infectious Disease Service, The Peter Doherty Institute for Infection and Immunity, The Royal Melbourne Hospital, Melbourne, Victoria, Australia.
Jonathan R CarapetisWesfarmers Centre for Vaccines and Infectious Diseases, Telethon Kids Institute, University of Western Australia, Nedlands, Western Australia, Australia.
Asha C BowenWesfarmers Centre for Vaccines and Infectious Diseases, Telethon Kids Institute, University of Western Australia, Nedlands, Western Australia, Australia.

Funding

National Health and Medical Research Council Synergy Grant 2021/GNT2010716 (STARFISH)
6 · The paper itself

Abstract

Improving our understanding of superficial Streptococcus pyogenes (Strep A) carriage and transmission necessitates robust sampling methods. Here, we compared the effect of storing swab samples in fridge (+4°C) and freezer (-20°C) conditions on the recovery of laboratory-cultured S. pyogenes. Streptococcus pyogenes colony-forming units progressively declined at +4°C, but not at -20°C, over 2 weeks. Results demonstrate that freezing is preferable over refrigeration for storage and transport of skin and throat swabs to ensure that culturing accurately reflects the true results of sampling. This is particularly important in remote community research and practice when immediate incubation is not possible or batch processing is most practical, increasing the elapsed time between collection and laboratory analysis. The study suggests that temperature negatively affects S. pyogenes viability and provides a method to further investigate the role of other environmental factors affecting S. pyogenes transmission.

Indexed as

Specimen HandlingStreptococcus pyogenesBacteriological TechniquesHumansMicrobial ViabilityPharynxSkinStreptococcal InfectionsTemperature

Identifiers

PMID39517098
PMCPMC11549027

What OpenQuestion holds

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LicenceCC BY
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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.